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高效液相色谱法测定肝泰胶囊中水飞蓟宾的含量
引用本文:王雯佶,张纯,檀密艳,高申.高效液相色谱法测定肝泰胶囊中水飞蓟宾的含量[J].中国药房,2006,17(22):1736-1737.
作者姓名:王雯佶  张纯  檀密艳  高申
作者单位:1. 第二军医大学长征医院药学部,上海市,200003
2. 第二军医大学药学院,上海市,200433
摘    要:目的建立以高效液相色谱法测定肝泰胶囊中水飞蓟宾含量的方法。方法色谱柱为DiamonsilC18,流动相为磷酸二氢钾-甲醇(45∶55),流速为1·0ml/min,检测波长为288nm,内标为山奈酚,柱温为(25±2)℃,进样量为20μl。结果水飞蓟宾检测浓度在0·126~1·26mg/ml范围内线性关系良好(r=0·9998),平均加样回收率为102·9%(RSD=1·50%)。结论本方法简便、准确、重现性好,可用于本品的含量测定。

关 键 词:高效液相色谱法  肝泰胶囊  水飞蓟宾  含量
文章编号:1001-0408(2006)22-1736-02
收稿时间:01 23 2006 12:00AM
修稿时间:02 23 2006 12:00AM

Assaying of Silybin in Gantai Capsule by HPLC
WANG Wenji,ZHANG Chun,TAN Miyan,GAO Shen.Assaying of Silybin in Gantai Capsule by HPLC[J].China Pharmacy,2006,17(22):1736-1737.
Authors:WANG Wenji  ZHANG Chun  TAN Miyan  GAO Shen
Abstract:OBJECTIVE:To establish an HPLC method for the assaying of silybin in Gantai capsule.METHODS:The assay was performed on Diamonsil C18 column.The mobile phase consisted of potassium dihydrogen phosphate solution-methanol(45:55)with a flow rate at 1.0ml/min,detection wavelength at 288nm,column temperature at(25±2)℃.The sample size was 20μl and the internal standard was kaempferol.RESULTS:There was a good linear relationship within the concentration range of 0.126~1.26 mg/ml(r=0.9 998)for silybin.The average recovery was 102.9%(RSD=1.50%).CONCLUSION:The method was simple,accurate and reproducible,and suitable for the assaying of silybin in Gantai capsule.
Keywords:HPLC  Gantai capsule  Silybin  Content
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