| HPLC法测定α-细辛脑PLGA原位凝胶中α-细辛脑的含量 |
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| 引用本文： | 罗玉玲,张永萍,梁光义.HPLC法测定α-细辛脑PLGA原位凝胶中α-细辛脑的含量[J].中国药房,2012(19):1770-1772. |
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| 作者姓名： | 罗玉玲 张永萍 梁光义 |
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| 作者单位： | 贵阳中医学院 |
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| 基金项目： | 贵州省优秀科技教育人才省长专项资金项目(黔省专合字(2009)87号);贵阳中医学院研究生教育创新计划项目(ZYYCX10024) |
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| 摘 要： | 目的:建立测定α-细辛脑聚乳酸-羟基乙酸共聚物(PLGA)原位凝胶中α-细辛脑含量的方法。方法:采用高效液相色谱法。色谱柱为SHIM-PACK VP-ODS(250mm×4.6mm,5μm),流动相为甲醇-水(75:25),检测波长为258nm,流速为1.0mL·min-1,柱温为30℃。结果:α-细辛脑的检测浓度在10.4～104.0μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9999);平均加样回收率为95.83%,RSD=2.47%(n=9)。结论:本方法准确、可靠、稳定,可用于α-细辛脑PLGA原位凝胶的质量控制。
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| 关 键 词： | 针灸 埋植材料 α-细辛脑 聚乳酸-羟基乙酸共聚物原位凝胶 含量测定 高效液相色谱法 |
Content Determination of α-asarone in PLGA in-situ Gel by HPLC |
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| LUO Yu-ling,ZHANG Yong-ping,LIANG Guang-yi.Content Determination of α-asarone in PLGA in-situ Gel by HPLC[J].China Pharmacy,2012(19):1770-1772. |
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| Authors: | LUO Yu-ling ZHANG Yong-ping LIANG Guang-yi |
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| Institution: | (Guiyang College of Traditional Chinese Medicine,Guiyang 550002,China) |
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| Abstract: | OBJECTIVE:To establish a method for the content determination of α-asarone in polylactic-co-glycolic acid (PLGA) in-situ gel.METHODS:HPLC method was adopted.The determination was performed on SHIM-PACK VP-ODS(250 mm×4.6 mm,5 μm)column with mobile phase consisted of methanol-water(75:25)at the flow rate of 1.0 mL·min-1.The detection wavelength was set at 258 nm and column temperature was 30 ℃.RESULTS:The linear range of α-asarone was 10.4～104.0 μg·mL-1(r=0.999 9) with an average recovery of 95.83%(RSD=2.47%,n=9).CONCLUSION:The established method is simple,accurate and reliable.It is fit for quality control of PLGA in-situ gel. |
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| Keywords: | Acupuncture and moxibustion Material for planted α-asarone PLGA in-situ-gel Content determination HPLC |
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