水蛭醇提物与水提物对肺缺血再灌注模型大鼠的保护作用

目的：研究水蛭醇提物与水提物对肺缺血再灌注模型大鼠的保护作用，并比较两者的作用效果。方法：夹闭阻断大鼠左肺门30min，开放后再灌注120min以复制肺缺血再灌注损伤模型。32只SD大鼠随机分为假手术（等容生理盐水）组、模型（等容生理盐水）组、水蛭醇提物（400mg／kg）组、水蛭水提物（400mg／kg）组。于术前3d腹腔注射给药，每天1次，连续3d。放射免疫法测定大鼠血浆血栓素B2（TXB2）和6酮前列腺素F1a（6-keto-PGF1a）含量可反映血栓素A2（TXA2）、前列环素（PGI2）水平]；酶联免疫吸附法测定大鼠血清一氧化氮（NO）含量与一氧化氮合酶（NOS）活性。结果：与假手术组比较，模型组大鼠血浆中TXB2含量显著增加，TXB2／6．keto-PGF1a比值显著降低，血清中NO含量显著增加，NOs活性显著增强（P〈0．01或P〈0．05）；与模型组比较，水蛭醇提物组、水蛭水提物组大鼠血浆TXB2、6-keto-PGF1a含量显著减少，TXB2／6．keto—PGF1a比值显著升高，血清N0含量显著减少，NOS活性显著减弱（P〈0．01或P〈0．05），且以上指标水蛭醇提物组均好于水蛭水提物组，但差异无统计学意义（P〉0．05）。结论：水蛭醇提物与水蛭水提物均可降低肺缺血再灌注模型大鼠TXA2、PGI2、NO含量和NOS活性，且前者效果好于后者。

Protective Effects of Ethanol Extract and Water Extract of Whitmania acranulata on Lung Ischemia-reperfu- sion Injury Model Rats

OBJECTIVE： To study and compare the protective effects of ethanol extract vs. water extract of Whitmania acranulata lung ischemia-reperfusion injury model rats. METHODS： Lung ischemia-reperfusion injury model was induced by 120 reperfusion following 30 min hilum of left lung occlusion and blocking-up. 32 SD rats were randomly divided into sham operation group （constant volume of normal saline）, model group （constant volume of normal saline）, ethanol extract of A. gulo group （400 mg/ kg）, water extract of W. acranulata group （400 mg/kg）. They were given intraperitoneal injection of medicine 3 clays before surgery once a day, for 3 consecutive days. Radioimmunoassay was adopted to determine the contents of TXB2 and 6-keto-PGFla to reflect the plasma level of TXA2 and PGI2]; ELISA assay was used to detect NO content and NOS activity. RESULTS： Compared with sham operation group, the contents of TXB2 and 6-keto-PGF1a, serum content of NO, the activity of NOS in plasma were in- creased significantly in model control group, meanwhile, TXB12/6-keto-PGFla ratio was decreased （P〈0.01 or P〈0.05）. Compared with model group, the plasma contents of TXB2 and 6-keto-PGFla, serum content of NO, the activity of NOS were decreased significantly in ethanol extract of W. acranulata group and water extract of W. acranulata group （P〈0.01 or P〈0.05） ;meanwhile, TXB2/6-keto-PGFla ratio was increased, above index of ethanol extract of W. acranulata group were better than those of water extract of W. acranulata group. CONCLUSIONS： The ethanol extract of W. acranulata group and water extract of W. acranulata group can reduce the contents of TXA2, PGI2 and NO, NOS activity in lung ischemia-reperfusion injury rats, and the former is bet- ter than the latter.