| 阿特拉津及其代谢物在大鼠尿液中的排泄动力学研究 |
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| 引用本文： | 梁秋云,谭珍媛,黄慧学,陈莉,徐宏珍.阿特拉津及其代谢物在大鼠尿液中的排泄动力学研究[J].中国药房,2011(21):1929-1932. |
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| 作者姓名： | 梁秋云 谭珍媛 黄慧学 陈莉 徐宏珍 |
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| 作者单位： | 广西医科大学药学院;广西中医学院药学院; |
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| 基金项目： | 广西壮族自治区教育厅科研项目(200911MS135) |
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| 摘 要： | 目的:建立大鼠尿液中阿特拉津(ATR)及其代谢物脱乙基阿特拉津(DEA)、脱异丙基阿特拉津(DIA)及脱乙基脱异丙基阿特拉津(DEDIA)的分析方法,并考察其排泄特征。方法:采用乙腈沉淀蛋白、SFC柱富集等对尿液样品预处理;以高效液相色谱法测定大鼠尿液中ATR及其代谢物的含量,色谱柱为WelchromTM C18,流动相为0.5%冰醋酸甲醇液-0.5%冰醋酸水溶液梯度洗脱,检测波长为225nm;大鼠按25、50、125mg·kg-1灌服给予ATR溶液,于给药前和给药后6、12、18、24、48、72h收集尿液测定并计算其72h累积排泄量占给药量比。结果:ATR、DEA、DIA、DEDIA检测浓度线性范围分别为0.5～50、1～100、1～100、15～1500μg·mL-(1r=0.994～0.998),各样本高、中、低浓度的回收率为96.76%～99.03%,日内、日间RSD均<5%;大鼠灌服ATR后,尿样中主要以代谢物DEDIA为主(>75%),而原型、DEA和DIA排泄量均较少(<10%)。结论:所建立的分析方法准确、灵敏、重复性好,适用于尿液中ATR及其代谢物的分析;ATR在大鼠体内以代谢物DEDIA排泄占绝对优势,降解更趋完全。
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| 关 键 词： | 阿特拉津 代谢物 大鼠 尿液 排泄动力学 |
Excretion Kinetics Study of Atrazine and Its Metabolites in Rat Urine |
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| LIANG Qiu-yun,TAN Zhen-yuan,HUANG Hui-xue HUANG Hui-xue,CHEN Li,XU Hong-zhen.Excretion Kinetics Study of Atrazine and Its Metabolites in Rat Urine[J].China Pharmacy,2011(21):1929-1932. |
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| Authors: | LIANG Qiu-yun TAN Zhen-yuan HUANG Hui-xue HUANG Hui-xue CHEN Li XU Hong-zhen |
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| Institution: | LIANG Qiu-yun,TAN Zhen-yuan,HUANG Hui-xue(School of Pharmaceutical Science,Guangxi Medical University,Nanning 530021,China) HUANG Hui-xue,CHEN Li,XU Hong-zhen(School of Pharmaceutical Science,Guangxi Traditional Chinese Medical University,Nanning 530001,China) |
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| Abstract: | OBJECTIVE:To establish a method for the analysis of atrazine(ATR) and its metabolites EDA,DIA and DEDIA in rat urine,and to investigate the excretion characteristics. METHODS:Sample preparation involved protein precipitation with acetonitrile and extraction with Silico Flash Column before HPLC method was used to determine the content of ATR and its metabolites. The determination was perfomred on WelchromTM C18 column with mobile phase consisted of 0.5% glacial acetic acid methanol-0.5% glacial acetic acid(g... |
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| Keywords: | Atrazine Metabolites Rat Urine Excretion kinetics |
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