正交试验优选二氢青蒿素脂质体的制备工艺

目的:优选二氢青蒿素脂质体的制备工艺。方法:以大豆磷脂与胆固醇的质量比、脂质与药物的质量比、水合溶液的种类为考察因素,以包封率为评价指标,利用正交试验优选制备工艺。结果:优选的工艺为采用薄膜-超声法,大豆磷脂与胆固醇的质量比为5∶1,脂质与药物的质量比为5∶1,以0.9%NaCl注射液为水合溶液。制得的脂质体形态均匀,包封率>85%。结论:该制备工艺和处方可以得到高包封率和稳定的二氢青蒿素脂质体。

Optimization of the Preparation Technology of Dihydroartemisinin Liposome by Orthogonal Test

OBJECTIVE：To optimize the preparation technology of dihydroartemisinin liposome.METHODS：The preparation technology of dihydroartemisinin liposomes was optimized by orthogonal test with ratio of granulesten to cholesterol,ratio of lipid to drugs and types of aqueous solution as factors and using encapsulation efficiency as index.RESULTS：Film-ultrasound method was used to optimize the preparation method.The optimal preparation technology was as follows：ratio of granulesten to cholesterol was 5：1,ratio of lipid to drugs was 5：1 and 0.9% NaCl injection was used as aqueous solution.Prepared liposome was uniform in shape with encapsulation efficiency more than 85%.CONCLUSION：The method can be used to prepare stable dihydroartemisinin liposome with high encapsulation efficiency.