维生素C和维生素E单用及联用对慢性酒精性肝损伤模型大鼠的预防性保护作用

目的:观察维生素C(VC)和维生素E(VE)单用及联用对慢性酒精性肝损伤模型大鼠的预防性保护作用。方法:取60只大鼠,随机均分为对照组、模型组、VC组(150mg·kg-1)、VE组(250mg·kg-1)和同剂量联合用药组(VC+VE组),除对照组给予等量生理盐水灌胃外,其余各组连续灌胃56°白酒建立慢性酒精性肝损伤模型,同时给予相应药物,每天1次,6周后检测各组大鼠血清丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST),肝匀浆中超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量,并观察肝组织病理学变化,免疫组织化学法检测肝组织中Ⅰ和Ⅲ型胶原的表达情况。结果:与模型组比较,VC组、VE组、VC+VE组ALT和AST活性、MDA含量、Ⅰ和Ⅲ型胶原表达均显著性降低(P<0.05),SOD活性显著性升高(P<0.05),肝脏炎症反应和纤维化明显减轻,且单用组和联用组之间无显著性差异。结论:VC和VE可能通过清除自由基和抑制脂质过氧化过程对慢性酒精性肝损伤模型大鼠发挥预防性保护作用。

Preventive Protection Effects of Drug Combination of Vitamin C and Vitamin E or Alone on Chronic Alcoholic Liver Injury in Rats

OBJECTIVE： To observe the preventive protection effects of combination of vitamin C （VC） and vitamin E （VE） or alone on chronic alcoholic liver injury in rats. METHODS： 60 SD rats were randomly divided into control group, model group, VC group （150 mg·kg^-1）, VE group （250 mg·kg^-1） and VE＋VC group. Control group was given normal saline via i.g. gtt while other groups were given 56° liquor to establish chronic alcoholic liver injury model. Meanwhile, rats in VC, VE and VC＋VE groups were administered with corresponding drugs once a day. The serum levels of alanine aminotransferase （ALT）, aspartate aminotransferase （AST） and malondialdehyde （MDA） and the activity of superoxide dismutase （SOD） were determined after 6 weeks. Light microscope was applied to observe pathological change of liver tissue. The expression of collagen Ⅰ （Col Ⅰ） and collagen Ⅲ （Col Ⅲ ） were detected by immunohistochemistry. RESULTS： Compared with model group, in VC group, VE group and VC＋VE group the activity of ALT and AST, the content of MDA and the expression of Col Ⅰ and Col Ⅲ decreased significantly （P〈 0.05）, the activity of SOD increased significantly （P〈0.05）, the inflammation and fibrosis were relieved in pathological sections. There was no significant difference between VC group, VE group and VC＋VE group. CONCLUSION： VC and VE might have preventive protection effects on chronic alcoholic liver injury by scavenging radicals and inhibiting lipid peroxidation.