补阳还五汤对高脂血症模型大鼠血液流变学和血小板相关生物学指标的影响

目的:探讨补阳还五汤对于高脂血症模型大鼠血液流变学和血小板相关生物学指标的影响。方法:将雄性Wistar大鼠随机分为空白对照组(10只)和造模组(40只)。空白对照组大鼠给予普通饲料,造模组大鼠给予高脂饲料,分组喂养至少6周以复制高脂血症模型。将造模成功的大鼠随机分为模型对照组、阳性对照组(辛伐他汀,0.004 g/kg)和补阳还五汤低、高剂量组(3.5、14.0g/kg,以生药量计),每组10只。空白对照组和模型对照组大鼠灌胃生理盐水,各药物组大鼠灌胃相应药液,0.01 mL/g,每日1次,连续4周。末次给药30 min后,检测大鼠的血液流变学指标(全血黏度、血浆黏度)、血小板黏附作用相关指标黏附率、血管性血友病因子(vWF)、纤维连接蛋白(FN)]、血小板释放作用相关指标β-血小板球蛋白(β-TG)、血小板第4因子(PF4)]、血小板纤溶系统相关指标组织型纤溶酶原活化剂(t-PA)、纤溶酶原激活物抑制剂1(PAI-1)]、血小板参数血小板计数(PLT)、血小板分布宽度(PDW)、血小板平均体积(MPV)、血小板压积(PCT)、大型血小板比率(PLCR)]和凝血4项指标活化部分凝血活酶时间(APTT)、凝血酶时间(TT)、凝血酶原时间(PT)、纤维蛋白原(FIB)]。结果:与空白对照组比较,模型对照组大鼠全血黏度(低、中、高切变率)、血浆黏度、血小板黏附率以及vWF、FN、β-TG、PAI-1、PLT、MPV、PCT、PLCR、FIB含量或水平均显著升高(P<0.05或P<0.01),t-PA含量显著降低(P<0.05)。与模型对照组比较,各药物组大鼠全血黏度(除补阳还五汤高剂量组高切变率全血黏度外)、血浆黏度、血小板黏附率以及v WF、FN、β-TG、PAI-1、PLT、PDW(除补阳还五汤低、高剂量组外)、MPV、PCT、PLCR(除补阳还五汤低剂量组外)、FIB含量或水平均显著降低(P<0.05或P<0.01),t-PA含量(除阳性对照组外)均显著升高(P<0.05)。结论:补阳还五汤可能是通过降低血液黏度和FN含量、改善血小板黏附性、增强纤溶活性、改善血小板聚集状态、抑制凝血及血小板释放亢进等途径,来发挥对高脂血症模型大鼠病理状态的改善作用。

Effects of Buyang Huanwu Decoction on Hemorheology and Platelet Related Biological Indexes in Hyperlipidemia Model Rats

OBJECTIVE:To investigate the effects of Buyang huanwu decoction on hemorheology and platelet related biological indexes of hyperlipidemia model rats.METHODS:Male Wistar rats were randomly divided into blank control group(10 rats)and model group(40 rats).Blank control group was given normal diet,and model group was given high-lipid diet for 6 weeks at least to induce hypelipidemia model.After modeling,rats were randomly divided into model control group,positive control group(simvastatin,0.004 g/kg),Buyang huanwu decoction low-dose and medium-dose groups(3.5,14.0 g/kg,by crude drug),with 10 rats in each group.Blank control group and model control group were given normal saline intragastrically,administration groups were given relevant drug intragastrically,0.01 m L/g,once a day,for consecutive 4 weeks.Thirty min after last medication,hemorheological indexes(whole blood viscosity,plasma viscosity),platelet adhesion related indexesadhesion rate,von Willebrand factor(v WF),fibronectin(FN)],platelet release related indexesβ-thromboglobulin(β-TG),platelet factor 4(PF4)]and platelet fibrinolytic system related indexestissue plasminogen activator(t-PA),plasminogen activator inhibitor(PAI-1)],platelet parameters(PLT,PDW,MPV,PCT,PLCR),4 kinds of coagulation parameters(APTT,TT,PT,FIB)were detected.RESULTS:Compared with blank control group,the whole blood viscosity(low,medium and high shear rate),plasma viscosity,platelet adhesion rate,the contents or levels of vWF,FN,β-TG,PAI-1,PLT,MPV,PCT,PLCR and FIB in model control group were increased significantly(P<0.05 or P<0.01),and t-PA content was significantly decreased(P<0.05).Compared with model control group,the whole blood viscosity(except for whole blood viscosity of high shear rate in Buyang huanwu decoction high-dose group),plasma viscosity,platelet adhesion rate,the contents or levels of vWF,FN,β-TG,PAI-1,PLT,PDW(except for Buyang huanwu decoction low-dose and high-dose groups),MPV,PCT,PLCR(except for Byang huanwu decoction low-dose group)and FIB were decreased significantly(P<0.05 or P<0.01),while t-PA content(except for positive control group)was increased significantly(P<0.05).CONCLUSIONS:Buyang huanwu decoction can significantly improve the pathological state in hyperlipidemia model rats by reducing blood viscosity and FN content,improving platelet adhesion,enhancing fibrinolytic activity,improving platelet aggregation,inhibiting hypercoagulability and hyperplatelet release.