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| 黄芩中黄芩苷提取工艺的研究 | |
| 引用本文： | 聂继红,王萍,孙蕾,赵哲,加孜依拉.黄芩中黄芩苷提取工艺的研究[J].中国药房,2005,16(14):1051-1053. |
| 作者姓名： | 聂继红 王萍 孙蕾 赵哲 加孜依拉 |
| 作者单位： | 1. 新疆医科大学附属中医医院药学部,乌鲁木齐市,830000 2. 新疆医科大学中医学院,乌鲁木齐市,830000 3. 新疆医科大学药学院,乌鲁木齐市,830000 |
| 基金项目： | 新疆科学研究与技术开发计划 |
| 摘 要： | 目的:建立黄芩提取液中黄芩苷的含量测定方法,优选黄芩苷的提取工艺。方法:以黄芩苷为指标,采用高效液相色谱法测定黄芩苷含量;采用单因素试验与正交试验优选出黄芩苷的提取工艺。结果:黄芩苷检测浓度在0.05912～0.02956mg/ml范围内线性关系良好(r=0.99974),平均回收率为99.5%,RSD=1.10%(n=5);优选的提取工艺为以14倍药材量的水回流提取2次,每次1h。结论:建立的含量测定方法简便,优选出的提取工艺稳定、合理、可行。 |
| 关 键 词： | 黄芩苷 含量测定 提取工艺 单因素试验 正交试验 |
| 文章编号： | 1001-0408(2005)14-1051-03 |
| 修稿时间： | 2004年12月28 |
Study on Extraction of Baicalin from Scutellaria Baicalensis Georgi |
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| NIE Jihong,WANG Ping,SUN Lei,ZHAO Zhe,JIA Ziyila.Study on Extraction of Baicalin from Scutellaria Baicalensis Georgi[J].China Pharmacy,2005,16(14):1051-1053. | |
| Authors: | NIE Jihong WANG Ping SUN Lei ZHAO Zhe JIA Ziyila |
| Abstract: | OBJECTIVE:To establish a method for content determination of baicalin in Scutellaria baicalensis Georgi extract and to optimize the extraction technique.METHODS:The content of baicalin was adopted as index and was deterˉmined by HPLC;Single factor test and orthogonal test were employed to optimize the extraction technique.RESULTS:The detected concentration of baicalin showed a good linear relation in the range of0.05912～0.02956mg/ml(r=0.99974),the average recovery was99.5%,RSD=1.10%(n=5);The optimal technique was twice recirculation in water solvent14times the amount of Scutellaria baicalensis Georgi flour,each time for1hour.CONCLUSION:The established method is conveˉnient,and the extraction technique is stable,reasonable and practicable. |
| Keywords: | Baicalin Content determination Extraction technique Single factor test Orthogonal test |
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