| HPLC法同时测定益气祛白颗粒中2种成分的含量 |
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| 引用本文： | 康雷,顾圣莹,朱冠华,杨秋娅,王玉珠,吴佳琪,李晓宇,刘皋林.HPLC法同时测定益气祛白颗粒中2种成分的含量[J].中国药房,2014(31):2918-2920. |
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| 作者姓名： | 康雷 顾圣莹 朱冠华 杨秋娅 王玉珠 吴佳琪 李晓宇 刘皋林 |
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| 作者单位： | 上海交通大学附属第一人民医院临床药学科,上海200082 |
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| 基金项目： | “重大新药创制”科技重大专项“十二五”项目子课题（No.20i1ZX09302-007-02）;上海市科研计划项目课题（No.09dZ1974600） |
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| 摘 要： | 目的:建立同时测定益气祛白颗粒中黄芪甲苷Ⅳ和木通皂苷D含量的方法。方法:采用高效液相色谱法。色谱柱为YMC-Pack ODS-AM(150 mm×4.6 mm,3μm),流动相为乙腈-水(梯度洗脱),流速为0.8 ml/min,检测波长为203 nm。结果:黄芪甲苷Ⅳ和木通皂苷D的质量浓度分别在10500、2.5500、2.5250μg/ml范围内与各自峰面积积分值呈良好的线性关系(r分别为0.999 9和0.999 7);平均加样回收率分别为106.09%、99.45%,RSD分别为1.81%、3.24%(n均为6)。结论:所建方法简单、稳定,结果准确、可靠,可为益气祛白颗粒的体内药动学研究提供依据。
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| 关 键 词： | 益气祛白颗粒 高效液相色谱法 含量测定 黄芪甲苷Ⅳ 木通皂苷D |
Simultaneous Determination of 2 Compounds in Yiqi Qubai Granules by HPLC |
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| KANG Lei,GU Sheng-ying,ZHU Guan-hua,YANG Qiu-ya,WANG Yu-zhu,WU Jia-qi,LI Xiao-yu,LIU Gao-lin.Simultaneous Determination of 2 Compounds in Yiqi Qubai Granules by HPLC[J].China Pharmacy,2014(31):2918-2920. |
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| Authors: | KANG Lei GU Sheng-ying ZHU Guan-hua YANG Qiu-ya WANG Yu-zhu WU Jia-qi LI Xiao-yu LIU Gao-lin |
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| Institution: | (Dept. of Clinical Pharmacy, The First Affiliated People＇s Hospital of Shanghai Jiaotong University, Shanghai 200082, China) |
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| Abstract: | OBJECTIVE： To develop a method for simultaneous determination of astragaloside Ⅳ and akebia saponin D in Yiqi Qubai granules. METHODS： HPLC method was adopted. The determination was performed on a YMC-Pack ODS-AM （150 min×4.6mm, 3μm） column with mobile phase consisted of acetonitrile-water （gradient elution） at the flow rate of 0.8 ml/min. The detection wavelength was set at 203 nm. RESULTS： The linear ranges were 10-500 μg/ml for astragaloside Ⅳ （r：0.999 9） and 2.5-250 μg/ml for akebia saponin D （r= 0.999 7）. The average recoveries were 106.09 % （RSD： 1.81%, n= 6） and 99.45 % （RSD= 3.24%, n=6）, respectively. CONCLUSIONS： The established method is convenient, stable, accurate and reliable, and can provide reference for pharmacokinetic study of Yiqi qubai granules in vivo. |
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| Keywords: | Yiqi qubai granules HPLC Content determination Astragaloside Ⅳ Akebia saponin D |
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