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高效液相色谱法测定复方替硝唑漱口液中主药的含量
引用本文:钱方,黄伟,王雯佶,沈利民,吴洪,刘皋林.高效液相色谱法测定复方替硝唑漱口液中主药的含量[J].中国药房,2003,14(5):298-299.
作者姓名:钱方  黄伟  王雯佶  沈利民  吴洪  刘皋林
作者单位:第二军医大学长征医院药学部,上海市,200003
摘    要:目的 :测定复方替硝唑漱口液中替硝唑和醋酸氯己定的含量。方法 :采用高效液相色谱法 ,以ODS为固定相 ,乙腈 -水(35∶65)为流动相 ,甲硝唑为内标,检测波长为254nm。结果 :替硝唑在0 01~0 10mg/ml浓度范围内线性关系良好(r=0 9999) ;醋酸氯己定在0 01~0 10mg/ml浓度范围内线性关系良好 (r=0 9997)。替硝唑和醋酸氯己定的平均回收率分别为97 06%~102 02 %、97 21%~103 40% ;日内差分别为0 20%~1 26 %、0 71 %~1 11% ,日间差分别为0 50 %~2 06 %、0 80%~2 17%。结论 :本法可用于测定替硝唑、醋酸氯己定在各种制剂中的含量。

关 键 词:替硝唑  醋酸氯己定  复方替硝唑漱口液  高效液相色谱法
文章编号:1001-0408(2003)05-0298-02
修稿时间:2002年4月23日

Determination of the Contents of the Main Components in Co- tinidazole Gargle by HPLC
QIAN Fang,HUANG Wei,WANG Wenji,SHENG Limin,WU Hong,LIU Gaolin.Determination of the Contents of the Main Components in Co- tinidazole Gargle by HPLC[J].China Pharmacy,2003,14(5):298-299.
Authors:QIAN Fang  HUANG Wei  WANG Wenji  SHENG Limin  WU Hong  LIU Gaolin
Abstract:OBJECTIVE:To develop a HPLC method for determination of tinidazole and chlorhexidine acetate in co-tinidazole gargle.METHODS:Tinidazole and chlorhexidine acetate in co-tinidazole gargle were determined by RP-HPLC with metronidazole as the internal standard.ODS was adopted as stationary phase and acetonitrile-water(35∶65)as mobile phase.The detector was operated at UV254nm.RESULTS:The calibration curve of tinidazole was linear within the concenˉtration range of(0.01~0.10)mg/ml(r=0.9999);The calibration curve of chlorhexidine acetate was linear within the conˉcentration range of(0.01~0.10)mg/ml(r=0.9997).The average recoveries of tinidazole and chlorhexidine were(97.06~102.02)%and(97.21~103.40)%,respectively.The within-day coefficients of variance were(0.20~1.26)%and(0.71~1.11)%,respectively.The between-days coefficients of variance were(0.50~2.06)%and(0.80~2.17)%,respectiveˉly.CONCLUSION:The method is suitable for determination of preparations containing tinidazole and chlorhexidine acetate.
Keywords:tinidazole  chlorhexidine acetate  co-tinidazole gargle  HPLC
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