HPLC法测定连蒲胶囊中金丝桃苷的含量 |
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引用本文: | 黄丽,黄惠琼,李玲玲,肖玉琴.HPLC法测定连蒲胶囊中金丝桃苷的含量[J].中国药品标准,2011,12(5):368-370. |
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作者姓名: | 黄丽 黄惠琼 李玲玲 肖玉琴 |
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作者单位: | 1. 厦门市药品检验所,厦门,361012 2. 厦门中医院,厦门,361012 |
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摘 要: | 目的:建立测定连蒲胶囊中金丝桃苷含量的高效液相色谱法。方法:采用HPLC法,色谱柱为Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm);流动相为乙睛-0.5%磷酸溶液(16∶84);检测波长360 nm;流速1.0 mL.min-1。结果:金丝桃苷的进样量在0.026~1.037μg范围内线性关系良好(r=1.000 0),平均回收率为102.2%,RSD=0.4%(n=6)。结论:该方法灵敏、准确,重现性好,能有效地控制连蒲胶囊的内在质量。
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关 键 词: | 连蒲胶囊 高效液相色谱法 金丝桃苷 |
Determination of Hyperin in Lianpu Capsules by HPLC |
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Authors: | Huang Li Huang Hui-qiong Li Ling-ling and Xiao Yu-qing |
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Institution: | 1. Xiamen Institute of Drug Control, Xiamen 361012; 2. Xiamen Hosipital of TCM ,Xiamen 361012) |
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Abstract: | Objective: To establish a method of determining hyperin in Lian Pu Capsules. Methods :The content of hyperin was determined by HPLC method with a Agilent ZORBAX SB-Cts (4. 6 mm x 250 mm,5 μm) and a mobile phase of aeetonitrile-0. 5% phosphoric acid ( 16: 84). The detection wavelength was 360 nm. The flow rate was 1.0 mL·min-1. Results: The linear relation was good with the range of 0. 026 - 1. 037μg ( r = 1. 000 0 ). The average recovery was 102. 2% with RSD 0. 4% ( n = 6 ). Conclusion : The method was sensitive, accurate, reproducible and simple. It could be used in quality control of Lianpu Capsules. |
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Keywords: | Lianpu Capsules HPLC Hypefin |
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