牛黄解毒丸(大蜜丸)中黄芩苷的含量测定方法的改进 |
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引用本文: | 金丽鑫,徐愚聪,蒋畅.牛黄解毒丸(大蜜丸)中黄芩苷的含量测定方法的改进[J].中国药品标准,2012,13(4):275-278. |
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作者姓名: | 金丽鑫 徐愚聪 蒋畅 |
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作者单位: | 1. 成都中医药大学,成都,611130 2. 四川省食品药品检验所,成都,610097 |
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摘 要: | 目的:修订完善牛黄解毒丸现行标准中黄芩苷的含量测定方法。方法:参照《中国药典》2010年版黄芩苷含量测定的方法,优化了测定方法。采用Welch Matenals C18(4.6 mm×200 mm,5μm)色谱柱,甲醇-水-磷酸(45∶55∶0.2)为流动相,流量1.0 mL.min-1。结果:以进样量为横坐标,峰面积为纵坐标,建立回归方程Y=2 105.3X-3.577 7,r=0.999 8,黄芩苷在0.028 46~0.853 8μg范围内呈良好的线性关系,平均回收率为103.2%(n=6),RSD为1.29%。结论:修订了的含量测定方法科学、合理,重复性好,可用于该药的质量控制。
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关 键 词: | 牛黄解毒丸 黄芩苷 高效液相色谱法 含量 |
Determination of Baicalin in Niuhuang Jiedu Pills by HPLC |
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Authors: | Jin Lixin Xu Yucong and Jiang Chang |
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Institution: | 1. Chengdu University of TCM, Chengdu 611130;2. Sichuan Institue for Food and Drug Control, Chengdu 610097 ) |
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Abstract: | Objective: Revising and improving the existing standard of content determination of baicalin in Nihuang Jiedu Pills . Methods:Refer to the Chinese Pharmacopoeia 2010 edition of baicalin content determination , improve the method. HPLC separation was achieved on welch matenals C la (4. 6 mm × 250 mm,5 μm). The mobile phase was methanol-water-phosphoric acid (45:55:0. 2) at the flow rate of 1.0 mL .min^-1. Results: The regression equation Y=2 105. 3X -3. 577 7, r =0. 999 8; results showed that baicalin in the range of 0. 028 46 -0. 853 8 μg was in good linear relationship. The average recovery was 103.2% (n =6) , and RSD was 1.29%. Conclusion: The revised method for the determination of baicalin in Nihuang Jiedu Pills was scientific, rational, feasible, in good reproducibility, and can be used for quality control of Niuhuang Jiedu Pills |
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Keywords: | Niuhuang Jiedu Pills baicalin HPLC assay |
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