肾石通丸质量标准的研究 |
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引用本文: | 李虹,郭宏伟,沈书博.肾石通丸质量标准的研究[J].中国药品标准,2012,13(4):268-271. |
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作者姓名: | 李虹 郭宏伟 沈书博 |
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作者单位: | 辽宁省食品药品检验所,沈阳,110023 |
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摘 要: | 目的:研究肾石通丸的质量标准。方法:薄层鉴别木香、鸡内金、金钱草;RP-HPLC法测定丹参中丹酚酸B的含量。含量测定采用Waters C18色谱柱(4.6 mm×200 mm,5μm),流动相为甲醇-乙腈-甲酸-水(30∶10∶1∶59),流量1 mL.min-1,检测波长286 nm。结果:薄层鉴别阴性对照均无干扰;含量测定丹酚酸B在0.054 67~2.186 69μg范围内有良好的线性关系(r=0.999 9),平均加样回收率为99.0%(n=6)。结论:所建方法简单、可靠,可用于肾石通丸的质量控制。
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关 键 词: | 去氢木香内酯 鸡内金 山柰素 槲皮素 金钱草 丹酚酸B 肾石通丸 高效液相色谱法 |
Study on Quality Standard for Shenshitong Pills |
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Authors: | Li Hong Guo Hongwei and Shen Shubo |
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Institution: | (Liaoning Institute for Food and Drug Control , Shenyang 110023 ) |
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Abstract: | Objective: To establish a method for the quality standard of Shenshitong Pills. Methods: The TLC methods were established for Aucklandiae Radix ,Galli Gigerii Endothelium Corneum, Lysimachiae Herba and an RP-HPLC methed was established for the deter mination of salvianolic acid B in Shenshitong Pills. Waters Cis (4.6 mm× 200 mm ,5 μm) colmnn was used and methanol-acetonitrile-methanoic acid-water (30: 10: 1: 59) was as the mobile phase at flow rate of 1.0 mL . min^-1. The detection wavelength was 286 nm. Results:TCL results displayed that negative control was non-interference. The linear range of salvianolic acid B was 0. 054 67- 2. 186 69 ~g( r = 0. 999 9) , the main recovery (n = 6)was 99. 0%. Conclusion: The method is convenient, reliable and suitable for quality assessment of Shengshitong Pills . |
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Keywords: | dehydrocostuslactone Galli Gigerii Endothelium Corneum kaempferol quercetin Lysimachiae Herba salvianolic acid B Shengshitong Pills HPLC |
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