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HPLC法测定复方桔梗止咳片中甘草苷和甘草酸含量
引用本文:邰顺章,袁步娟,黄柳燕.HPLC法测定复方桔梗止咳片中甘草苷和甘草酸含量[J].药学与临床研究,2014,22(2):115-117.
作者姓名:邰顺章  袁步娟  黄柳燕
作者单位:邰顺章 (盐城市药品检验所,盐城,224002); 袁步娟 (盐城市药品检验所,盐城,224002); 黄柳燕 (盐城师范学院,盐城,224002);
摘    要:目的:建立同时测定复方桔梗止咳片中甘草苷和甘草酸含量的HPLC检测方法。方法:采用Agilent C18(4.6 mm×150 mm,5μm)色谱柱;以乙腈-0.05%磷酸为流动相进行梯度洗脱;流速1.0 mL·min-1;检测波长237 nm;进样量:10μL;柱温30℃。结果:甘草苷、甘草酸分别在10.65~42.60 mg·L-1、97.30~389.30 mg·L-1范围内呈良好的线性关系,r分别为1.0000、1.0000;平均回收率(n=6)分别为98.80%(RSD=0.73%)、99.84%(RSD=0.10%)。测得6批复方桔梗止咳片,每片分别含甘草苷135.28μg、123.16μg、113.21μg、136.08μg、160.84μg、156.32μg,甘草酸441.36μg、405.86μg、364.69μg、446.93μg、518.22μg、509.65μg。结论:该方法简便,重复性好,可用于同时测定复方桔梗止咳片中甘草苷和甘草酸的含量。

关 键 词:复方桔梗止咳片  高效液相色谱法  甘草苷  甘草酸  含量测定
收稿时间:2013/12/18 0:00:00
修稿时间:2014/2/12 0:00:00

HPLC Method for Determination of Liquorice Glycosides and Glycyrrhizic acid Content in the Compound Radix Platycodi Cough Tablets
TAI Shun-zhang,YUAN Bu-juan and HUANG Liu-yan.HPLC Method for Determination of Liquorice Glycosides and Glycyrrhizic acid Content in the Compound Radix Platycodi Cough Tablets[J].Pharmacertical and Clinical Research,2014,22(2):115-117.
Authors:TAI Shun-zhang  YUAN Bu-juan and HUANG Liu-yan
Institution:1 yancheng Institute for Drug Control, Yancheng 224002; 2yancheng Normal College, Yancheng 224002
Abstract:Objective:To establish a quantitative method for the determination of licorice glycosides and glycyrrhizic acid in the compound radix platycodi cough tablets by high-performance liquid chromatography(HPLC) coupled with ultraviolet detection.Methods:An Agilent C18column(4.6 mm×150 mm,5 μm) was adopted.The mobile phase consisted of a mixture of acetonitrile-0.05% phosphoric acid in gradient elution at a flow rate of 1.0 mL·min-1,the detection wavelength was set at 237 nm;The sampling volume was 10 μL;The column temperature was 30 ℃.Results:The calibration curve showed a good linearity in the range of 10.65~42.60 mg·L-1(r=1.0000) for licorice glycosides and 97.30~389.30 mg·L-1(r=1.0000) for glycyrrhizic acid,respectively.The average recoveries(n=6) of licorice glycosides and glycyrrhizic acid were 98.80%(RSD=0.73%) and 99.84%(RSD=0.10%),respectively.Six batches of compound radix platycodi cough tablets were measured and the content per pill was respectively as follows:liquorice glycosides,135.28 μg,123.16 μg,113.21 μg,136.08 μg,160.84 μg and 156.32 μg;glycyrrhizic acid,441.36 μg,405.86 μg,364.69 μg,446.93 μg,518.22 μg and 509.65 μg.Conclusions:The developed method is simple and reproducible,which can be used for the quantitative determination of licorice glycosides and glycyrrhizic acid in the compound radix platycodi cough tablets.
Keywords:Compound radix platycodi cough tablets  Licorice glycosides  Glycyrrhizic acid  Content determination  Assay
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