| HPLC法测定消癌平注射液中5种酚酸的含量 |
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| 引用本文： | 张阿琴,张 仓,梁敬钰.HPLC法测定消癌平注射液中5种酚酸的含量[J].药学与临床研究,2011,19(6):498-500. |
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| 作者姓名： | 张阿琴 张 仓 梁敬钰 |
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| 作者单位： | 1. 中国药科大学中药学院天然药物化学教研室,南京,210009
2. 南京圣和药业有限公司,南京,210038 |
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| 基金项目： | 国家"重大新药创新"科技重大专项资助项目 |
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| 摘 要： | 目的:建立HPLC法测定消癌平注射液中5种酚酸含量的方法,为其质量控制提供依据.方法:采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm),0.1％甲酸溶液-0.1％甲酸乙腈溶液为流动相,梯度洗脱,检测波长300 nm.结果:5种酚酸(绿原酸、新绿原酸、隐绿原酸、香草酸和对香豆酸)在标准曲线范围内均有较好的线性范围(r2＞0.9999),且该方法具有良好的精密度、重复性和准确度;应用该方法同时测定了25批消癌平注射液中的5种酚酸的含量.结论:本检测方法简便、准确、可靠,为消癌平注射液中酚酸类成分的质量控制提供了依据.
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| 关 键 词： | HPLC法 消癌平注射液 酚酸 |
| 收稿时间： | 2011/8/18 0:00:00 |
| 修稿时间： | 2011/9/16 0:00:00 |
HPLC Analysis of Five Phenolic Acids in Xiaoaiping Injection |
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| ZHANG A-qin,ZHANG Cang and LIANG Jing-yu.HPLC Analysis of Five Phenolic Acids in Xiaoaiping Injection[J].Pharmacertical and Clinical Research,2011,19(6):498-500. |
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| Authors: | ZHANG A-qin ZHANG Cang and LIANG Jing-yu |
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| Institution: | ZHANG A-qin1,ZHANG Cang2,LIANG Jing-yu1 1Department of Natural Medicinal Chemistry,China Pharmaceutical University,Nanjing 210009,China,2Nanjing Sanhome pharmaceutical Co.,Ltd.,Nanjing 210038 |
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| Abstract: | Objective: To develop a simple and accurate HPLC-UV method for the simultaneous quantification of five phenolic acids in Xiaoaiping injection.Methods: Separations were performed on an Agilent ZORBAX SB-C18 column at 300 nm by gradient elution using 0.1% formic acid aqueous: acetonitrile.Results: Calibration curves presented good linear regression(r2>0.9999) within tested ranges.The precision and accuracy of the method were acceptable with RSD<0.50%,respectively,and overall recoveries of 97%~103% for the fiv... |
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| Keywords: | HPLC Xiaoaiping injection Phenolic acid |
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