血液标本培养5400例耐药性分析

目的分析血液标本培养中病原菌的种类和耐药情况。方法对5400份血培养标本结果进行回顾性分析，采用BACTEC9120全自动血培养仪进行血培养，分离出的病原菌采用Phoenix100全自动微生物鉴定系统进行细菌鉴定及耐药情况分析。结果5400份血培养标本中共检出细菌594株，血培养阳性检出率为11．0％；其中革兰阴性菌317株，占53．4％；革兰阳性菌261株，占43．9％；深部真菌16株，占2．7％。检出的主要病原菌为金黄色葡萄球菌、表皮葡萄球菌、大肠埃希菌、绿脓假单胞菌和肺炎克雷伯菌。主要革兰阳性球菌对万古霉素全部敏感。结论血培养分离的病原菌以革兰阴性菌为主，金黄色葡萄球菌和大肠埃希菌是败血症和菌血症主要病原菌，病原菌耐药情况严重。

Analysis of drug resistance in 5400 blood cultures

Objective To analyze the kinds of pathogenic bacteria and their drug resistance in the blood cul- ture from patients of our hospital. Methods The results of 5 400 specimens of blood culture in our hospital were ret- rospectively analyzed. The blood specimens were cultured by BACTEC 9120. The pathogenic bacteria were identified and their drug resistance were analyzed by Phoenix 100 auto microbiological identification systems. Results Among the 5 400 blood cultures,59d bacterial strains were detected, the detective rate was 11.0%. There were 317 strains of Gram-negative bacilli ,accounted for 53.4% ;261 strains of Gram-positive cocci, accounted for 43.9% ; 16 strains of deep fungi, accounted for 2.7%. The main pathogenic bacteria were Staphylococcus aureus, Staphylococcus epidermi- dis, Escherichia coli, Pseudomonas pyocyanea and Klebsiella pneumoniae. The sensitive rate of the main Gram-positive cocci to vancomycin was 100%. Conclusion Gram-negative bacilli are the main bacteria in blood culture. The main pathogenic bacteria of septicemia and bacteremia are Staphylococcus aureus and Escherichia coli. The drug resistance of pathogenic bacteria is very serious.