| 高效液相色谱法测定吲哚美辛缓释微球的含量 |
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| 引用本文： | 高颖,吴艺君,金蓉蓉,陆丹红,温俊,李文生.高效液相色谱法测定吲哚美辛缓释微球的含量[J].药物生物技术,2012(2):150-152. |
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| 作者姓名： | 高颖 吴艺君 金蓉蓉 陆丹红 温俊 李文生 |
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| 作者单位： | 温州医学院附属眼视光医院;温州医学院药学院 |
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| 基金项目： | 浙江省大学生科技创新活动计划(新苗人才计划)资助项目(No.2010R413051);浙江省医药卫生科学研究基金资助项目(No.2009A138);温州市科技局面上项目资助(No.Y20090014);温州医学院本专科学生科研立项资助课题(No.wyx201001007) |
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| 摘 要： | 建立吲哚美辛缓释微球中吲哚美辛含量的高效液相色谱方法。采用Zorbax Eclipse XDB-C18色谱柱(4.6 mm×150 mm,5μm),以pH 3.0的0.1 mol/L冰醋酸溶液-乙腈(20∶80)为流动相,流速为1.0 mL/min,柱温为30℃,检测波长为320 nm,进样量20μL,用外标法测得。吲哚美辛在2.0～80.0μg/mL范围内线性关系良好(r=0.999 9),平均回收率为98.70%,RSD为1.50%。本方法灵敏度高,操作简单可靠。
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| 关 键 词： | 高效液相色谱法 吲哚美辛 含量测定 |
HPLC Determination of Indomethacin Content in Indomethacin Microspheres |
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| GAO Ying,WU Yi-jun,JING Rong-rong,LU Dan-hong,WEN Jun,LI Wen-sheng.HPLC Determination of Indomethacin Content in Indomethacin Microspheres[J].Pharmaceutical Biotechnology,2012(2):150-152. |
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| Authors: | GAO Ying WU Yi-jun JING Rong-rong LU Dan-hong WEN Jun LI Wen-sheng |
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| Institution: | 1(1.Eye Hospital,School of Ophthalmology and Optometry,Wenzhou Medical College,Wenzhou 325027,China;2.School of Pharmacy,Wenzhou Medical College,Wenzhou,325027,China) |
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| Abstract: | Indomethacin microspheres were prepared by O/W solvent evaporation using PLGA and Eudragit RS 100,the mean diameters were 2 588.3±1 202.2 nm.A HPLC method for determiny indomethacin content in indomethacin microspheres was established.ZORBAX Eclipse XDB-C18 column(4.6 mm×150 mm,5 μm) was used with mobile phase consisting of 0.1mol/L glacial acetic acid(adjusted pH valne to 3.0)-acetonitrile(20∶80) and with a total flow rate of 1.0 mL/min.The column temperature was 30 °C.The UV detection wavelength was 320 nm,with injection amount 20 μL.The indomethacin curve was linear in the rangs of 2.0~80.0 μg/mL and the regression equation was Area=11.642×Amt+0.762,r=0.999 9.The average recovery of indomethacin was 98.70%,and the RSD was 1.50%.The method appeared to be highly sensitive,accurate and simple.It can be used to determine indomethacin content in indomethacin microspheres. |
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| Keywords: | HPLC Indomethacin Assay |
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