| 金针菇固态发酵产木聚糖酶的纯化及其性质研究 |
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| 引用本文： | 吴萍,李正鹏,何庆元,史均,盛伟,祝嫦巍.金针菇固态发酵产木聚糖酶的纯化及其性质研究[J].药物生物技术,2012(1):31-34. |
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| 作者姓名： | 吴萍 李正鹏 何庆元 史均 盛伟 祝嫦巍 |
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| 作者单位： | 安徽科技学院生命科学学院 |
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| 基金项目： | 安徽省教育厅自然科学基金(No.KJ2009B168Z);安徽科技学院生物学重点建设学科项目(No.AKXK2010 2-1) |
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| 摘 要： | 金针菇固态发酵产生木聚糖酶经硫酸铵盐析、透析、浓缩、Sephadex G-75分子筛层析等分离与纯化步骤,得到了一种相对分子质量约为59.7k的电泳纯木聚糖酶,其最终的活性收率为23.3%,纯化倍数为9.4。该木聚糖酶在pH值为6.0,50℃下的酶解效果最佳。酶的pH稳定性及热稳定性都比较好,在pH 4.0～8.0时基本稳定,80℃时相对酶活力仍保持在55.81%。Ca2+、Zn2+对该酶活性有一定的激活作用,而Hg2+、Cu2+则有较强的抑制作用。
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| 关 键 词： | 固态发酵 木聚糖酶 金针菇 纯化 性质 |
The purification and Characteristics of Xylanase from Flammulina Velutipes Solid-phase Fermentation |
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| WU Ping,LI Zheng-peng,HE Qing-yuan,SHI Jun,SHENG Wei,ZHU Chang-wei.The purification and Characteristics of Xylanase from Flammulina Velutipes Solid-phase Fermentation[J].Pharmaceutical Biotechnology,2012(1):31-34. |
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| Authors: | WU Ping LI Zheng-peng HE Qing-yuan SHI Jun SHENG Wei ZHU Chang-wei |
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| Institution: | (School of life science,Anhui University of Science and Technology,Fengyang 233100,China) |
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| Abstract: | The electrophoresis degree xylanase from flammulina velutipes solid-phase fermentation was purified by ammonium sulfate precipitation,dialysis,concentration,and Sephadex G-75 sieve chromatography.Its relative molecular mass is 59.7k.The recovery was 23.3% and 9.4 fold purification was achieved with better pH stability and thermal stability.The optimal enzymatic hydrolysis is obtained with pH 6.0 at 50 ℃,and is much more stable between pH 4.0~8.0.The relative enzyme activity is 55.81% at 80 ℃.Ca2+ and Zn2+ can stimulate the xylanase activity but Hg2+ and Cu2+ have strong inhibitory action. |
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| Keywords: | Solid-phase fermentation Xylanase Flammulina velutipes Purification Characteristics |
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