| 毛脉酸模内生真菌RGT-S11菌丝体化学成分研究 |
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| 引用本文： | 王博,王振月,王星星,郑成琳,卫丽曼.毛脉酸模内生真菌RGT-S11菌丝体化学成分研究[J].药物生物技术,2012(4):332-333,351. |
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| 作者姓名： | 王博 王振月 王星星 郑成琳 卫丽曼 |
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| 作者单位： | 黑龙江中医药大学药学院;黑龙江珍宝岛药业药物研究院;江苏康缘药业股份有限公司现代中药研究院妇科新药研究所 |
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| 基金项目： | 国家自然科学基金项目(No.30970300) |
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| 摘 要： | 研究毛脉酸模(Rumex gmelini Turcz.)内生真菌RGT-S11菌丝中的化学成分。运用溶剂萃取、硅胶柱色谱、ODS反相柱色谱、Sephadex LH-20柱色谱、制备液相等方法进行分离纯化,根据理化性质、NMR数据鉴定化合物的结构。结果:分离得到7个单体化合物:苯甲酸(Ⅰ),棕榈酸(Ⅱ),麦角甾-5,7,22-三烯-3β-醇(Ⅲ),油酸(Ⅳ),腺苷(Ⅴ),鸟苷(Ⅵ),没食子酸(Ⅶ)。以上化合物均为首次从内生真菌RGT-S11菌丝中分离得到。
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| 关 键 词： | 毛脉酸模 内生真菌 化学成分 提取分离 结构鉴定 相关性 |
Studies on Chemical Constituents of RGT-S11 Mycelium from Rumex gmelini Turcz |
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| Institution: | WANG Bo1,2,WANG Zhen-yue1,WANG Xing-xing1,3,ZHENG Cheng-lin1,WEI Lin-man1(1.Heilongjiang University of TCM;2.ZhenBaodao Institute of Pharmaceutical Research;3.Kanion Pharmaceutical Company) |
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| Abstract: | To study the chemical constituents of RGT-S11 mycelium from Rumex gmelini Turcz,various techniques,including silica gel column chromatography,ODS reversed phase chromatography column,Sephadex LH-20 chromatography column,preparative HPLC and method of recrystallization were applied to the separation of RGT-S11 mycelium.Seven compounds were isolated and identified as benzoic acid(Ⅰ),palmitic acid(Ⅱ),ergosta-5,7,22-trien-3β-ol(Ⅲ),oleinic acid(Ⅳ),adenosine(Ⅴ),guanosine(Ⅵ),gallic acid(Ⅶ) by 1H-NMR,13C-NMR,HMBC,HSQC,modern spectroscopy methods and so on.Compounds Ⅰ~Ⅵ were isolated from RGT-S11 mycelium for the first time.The further study on the isolation and structure identification of chemical constituents from the RGT-S4 can provide references for the pharmacodynamics research. |
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| Keywords: | Rumex gmelini Turcz Endophytic Fungi Chemistry constituents Extraction and separation Structure identification Correlation |
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