| 人组织型纤溶酶原激活剂(t-PA)在转基因烟草中的表达 |
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| 引用本文： | 齐拓荒,王庆华,刘进,奚涛.人组织型纤溶酶原激活剂(t-PA)在转基因烟草中的表达[J].药物生物技术,2006,13(5):322-325. |
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| 作者姓名： | 齐拓荒 王庆华 刘进 奚涛 |
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| 作者单位： | 中国药科大学生物技术中心,江苏,南京,210009 |
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| 摘 要： | 实验室构建了携带有人组织型纤溶酶原激活剂（tissue plasminogen activator，t-PA）植物表达载体并转化人根瘤农杆菌GV3101。该农杆菌以叶盘法转化烟草，建立表达t-PA的转基因烟草体系。通过对转化植株进行Southern blotting，RT-PCR，Western blotting和纤维蛋白琼脂糖平板（fibrin-agarose plate assay，FAPA）法检测，结果显示，在转基因烟草中成功地表达出有活性的人t-PA。
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| 关 键 词： | 人组织型纤溶酶原激活剂 转基因烟草 纤维蛋白琼脂糖平板法 免疫印记 |
| 文章编号： | 1005-8915（2006）05-0322-04 |
| 收稿时间： | 2006-03-23 |
| 修稿时间： | 2006-03-232006-04-18 |
Expression of Human Tissue -Type Plasminogen Activator in Transgenic Tobacco |
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| QI Tuo-huang,WANG Qing-hua,LIU Jin,XI Tao.Expression of Human Tissue -Type Plasminogen Activator in Transgenic Tobacco[J].Pharmaceutical Biotechnology,2006,13(5):322-325. |
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| Authors: | QI Tuo-huang WANG Qing-hua LIU Jin XI Tao |
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| Institution: | Research Center for Biotechnology, China Pharmaceutical University, Nanjing 210009,China |
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| Abstract: | The plant expressing vectors containing human t-PA gene was constructed and transformed in into agnobacterium GV3101.The transgenic tobacco system expressing human tissue plasminogen activator (t-PA) was established by infecting tobacco with the agnobacterium by leaf disk method.The transgenic efficiency was evaluated by Southern-blotting,RTPCR,Fibrin-agarose plate assay(FAPA) and Western-blotting.Results suggested that bio-active human t-PA was successfully expressed in the transgenic tobacco system. |
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| Keywords: | t-PA Transgenic tobacco FAPA Western-blotting |
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