α-细辛醚的体内抗运动性疲劳作用和对骨骼肌及脑组织自由基代谢的影响

目的 研究α-细辛醚的体内抗运动性疲劳作用和对骨骼肌及脑组织自由基代谢的影响.方法 将50只大鼠随机分成正常对照组,α-细辛醚组,运动组,运动＋α-细辛醚组和运动＋咖啡因组,每组10只.观察α-细辛醚对运动大鼠疲劳相关症状、跑台力竭时间和脑组织及骨骼肌组织SOD和MDA的影响.结果 实验第三周末运动组大鼠活动减少,毛发枯萎,无光泽,食欲减少.运动＋α-细辛醚组和运动＋咖啡因组大鼠与运动组比较,上述疲劳症状明显减轻.运动组大鼠脑组织和骨骼肌组织SOD活力分别为（26.63±7.85）NU/mg和（30.97±6.41）NU/mg均高于正常对照组（20.84±4.53）NU/mg和（24.21±3.74）NU/mg以及α-细辛醚组（21.12±3.38）NU/mg和（23.53±5.26）NU/mg;低于运动＋α-细辛醚组（32.64±8.35）NU/mg和（37.64±9.75）NU/mg（P＜0.05）;与运动＋咖啡因组（27.47±5.22）NU/mg和（32.22±6.89）NU/mg比较,差异无显著性（P＞0.05）.运动组大鼠脑组织和骨骼肌组织MDA含量分别为：（7.91±1.90）nmol/ml和（9.61±2.11）nmol/ml均高于正常对照组（2.73±1.13）nmol/ml和（3.89±1.35）nmol/ml、α-细辛醚组（2.71±1.03）nmol/ml和（3.77±1.45）nmol/ml和运动＋α-细辛醚组（5.23±2.01）nmol/ml和（6.17±1.57）nmol/ml（P＜0.05）;与运动＋咖啡因组（8.05±2.29）nmol/ml和（8.01±1.45）nmol/ml比较,差异无显著性（P＞0.05）.运动＋α-细辛醚组大鼠脑组织和骨骼肌组织SOD活力高于其余各组（P＜0.05）;而MDA含量低于其余各组,（P＜0.05）.结论 α-细辛醚具有较明显的抗运动性疲劳作用,其机理与α-细辛醚纠正疲劳机体自由基代谢失衡有关.

Effects of cis-asarone anti excise-induced fatigue to free radical metabolism in skeletal muscle and brain tissue

Objective Effects of cis-asarone from Acorus Tatarinowii Schott were investigated on anti excise-induced fatigue to free radicals metabolism in skeletal muscle and brain tissue of overtrained rats. Methods Fifty rats were divided into 5 groups: the control group, the cis-asarone group, the exercise group, the exercise plus cis-asarone group, the exercise plus caffeine group with ten rats in each group to study the effects of cis-asarone on fatigue-related symptoms, all-out time, superoxide dismutase (SOD) activity and malonyldialdehyde (MDA) levels of tissues including brain tissue and skeletal muscle of overtrained rats. Results After 3 weeks of experiment, the rats in the exercise group showed reduced activity, thinned fur, decreased appetite, while those symptoms significantly lessened in the rats of the exercise plus cis-asarone group and the exercise plus caffeine group. The all-out time of rats in the exercise plus cis-asarone group(82.53±11.40) min were significantly longer than those of the cis-asarone group (70.26±10.78 min), the exercise group(66.16±15.45 min)(P＜0.05),but they were significantly shorter than those of the exercise plus caffeine group (103.01±8.21 min) (P＜0.01).The SOD activity of the brain tissues (26.63±7.85) NU/mg and skeletal muscle (30.97±6.41) NU/mg in rats in the exercise group was significantly higher than that in the control group (20.84±4.53)NU/mg and (24.21±3.74)NU/mg], the cis-asarone group (32.64±8.35) NU/mg and (37.64±9.75) NU/mg], lower than the exercise plus cis-asarone group (32.64±8.35) NU/mg and (37.64±9.75) NU/mgl(P＜0.05).But there is no significant difference between the exercise group and the exercise plus caffeine group (27.47±5.22) NU/mg and (32.22±6.89) NU/mg](P＜0.05). The levels of MDA of the brain tissues (7.91±1.90) nmol/ml] and skeletal muscle (9.61±2.11) nmol/ml]in rats in the exercise group was significantly higher than those in the control group (2.73±1.13) nmol/ml and (3.89±1.35) nmol/mll, the cis-asarone group (2.71±1.03) nmol/ml and (3.77±1.45) nmol/ml]and the exercise plus cis-asarone group (5.23±2.01) nmol/ml and (6.17±1.57) nmol/mll (P＜0.05). But there is no significant difference between the exercise group and the exercise plus caffeine group (8.05±2.29) nmol/ml and (8.01±1.45) nmol/ml](P＞0.05). The SOD activity of the brain tissues and skeletal muscle in rats in the exercise plus cis-asarone group was significantly higher than those of the other groups (P＜0.05), while the levels of MDA were opposite (P＜0.05). Conclusion Cis-asarone was found to have significant effects on retarding fatigue, improving performance through high SOD activity,facilitating free radicals removal, and thus protecting the tissues from free radical damage.