体内外酒精对血清酶水平影响的实验观察

目的 探讨酒精在体内和体外对血清酶水平影响.方法 健康志愿者单次摄入200ml白酒后,分别于饮酒前0时与饮酒后0.5、1、2、3h时候,测定血中的碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、谷氨酰转肽酶(GGT)、肌酸激酶(CK)、淀粉酶(AMY)等酶水平.同时体外加入血清中微量酒精,也测定7种酶水平,以观察酒精对酶水平的影响.结果 健康志愿者摄入白酒后,酒精在体内可使血清中AST由高变低,其余酶水平均有不同程度的升高,但只有AMY水平升高有显著性意义(P＜0.01),体外酒精对血清酶均有一定程度抑制作用.结论 酒精在体内可使部份酶水平升高,但酒精对酶均有直接抑制作用,因此在日常血清酶学检验中,应避免酒精在体内外对等检血清的污染.

The Observation of Testing That Alcohol in Vivo and in Vitro Affects the Level of Sero-enzyme

Objective The way to discuss that alcohol in vivo or in vitro has effects on the level of sero- enzyme.Methods To test the levels of ALP, LDH, ALT, AST, GGT, CK, AMY in blood after healthy volunteers intake 200ml of distillate spirit in Master single test. And tests will be made in five phrases. The first phrase is just before drinking alcohol, and the rest four phrases are after drinking alcohol. They' re half an hour later, one hour later, two hours later, and three hours later. At the same time, to test the levels of seven enzymes after adding small volume of alcohol in blood in vitro, which is aimed to observe that whether or not alcohol has effects on enzymes. Results After health volunteers drank the distillate spirit, alcohol in vivo could change the AST in blood serum from high to low and other enzymes all rose in different levels. But only that the level of AMY stepped up had significant meaning. Besides, alcohol in vitro repressed the sero-enzymes in certain degree. Conclusion Alcohol in vivo could make the levels of some enzymes rose, but it also had direct repression to all the enzymes. In this sense, that to avoid the alcohol pollution to blood-serum which will be tested is necessary in usual sero-enzyme examination.