仙珠胶囊对去卵巢大鼠骨密度和血生化指标的影响

目的观察仙珠胶囊对去卵巢大鼠骨密度(BMD)、骨代谢标志物的影响。方法将去卵巢大鼠随机分为模型组,仙珠胶囊低、中、高剂量(2.715、5.430、10.860g生药/kg)组及阳性药倍美力片(0.104mg/kg)组。另设假手术组。连续ig给药3个月,测定血清中骨源性碱性磷酸酶(BALP)、Ⅰ型胶原交联羧基末端肽(CTX)、雌二醇(E2)、甲状旁腺素(PTH)、钙(Ca)、磷(P)的量,并剖取大鼠股骨测BMD。结果模型组大鼠BMD,E2、Ca、P的量低于假手术组(P<0.01);各给药组的这些指标高于模型组(P<0.05),高剂量仙珠胶囊组大鼠BMD高于阳性对照药(P<0.05)。模型组大鼠BALP、CTX、PTH高于假手术组(P<0.01),仙珠胶囊及倍美力组这3项指标均降低(P<0.01)。结论仙珠胶囊可有效增加去卵巢大鼠BMD,有效抑制骨丢失,增加成骨细胞活性,降低血清PTH水平,提高性激素水平。

Effect of Xianzhu Capsula on bone mineral density and blood biochemical indicators of ovariectomized rats

Abstract: Objective To observe the effect of Xianzhu Capsula on bone mineral density (BMD), bone metabolic indicators of ovariectomized rats. Methods Ovariectomized rats were divided into model group, Xianzhu Capsula lower, middle, and high dose (2.715, 5.430, and 10.860 g raw meterials/kg) groups, Premarin Tablets (0.104 mg/kg) group, and Sham group. The rats were ig administrated medicine continuously for three months. The bone alkaline phosphatase (BALP), collagen cross-linked carboxy-terminal peptide (CTX), estradiol (E2) , PTH ,Ca, and P in serum were detected; Meanwhile, thigh bones were dissected to measure BMD. Results BMD, E2, Ca ,and P content of the model rats were all significantly lower than those in Sham group (P<0.01), while those indicators of Premarin Tablets group and Xianzhu Capsula group were higher than those of model rats (P<0.05) , and BMD in high dose group of Xianzhu Capsula was higher than that in Premarin Tablets group (P<0.05). BALP, CTX, and PTH of model rats were all significantly higher than those in Sham group (P<0.01). BALP, CTX, and PTH in Premarin Tablets group and Xianzhu Capsula group decreased (P<0.01). Conclusion Xianzhu Capsula could obviously elevate BMD of ovariectomized rats and effectively improve hyper-transform statement. Meanwhile, it could decrease PTH and inhibit bone loss, and stimulate osteoblast activity and elevate gonadal hormone level.