银屑病（血热证）豚鼠复合模型的建立及评价研究

目的 基于中医血热证的病因，复合银屑病症状，建立银屑病病证结合的豚鼠复合模型。方法 采用多因素复合造模法，ig给予豚鼠复方热药造成血热证模型，豚鼠耳部皮肤涂抹5%盐酸普萘洛尔乳剂造成银屑病模型，从体征、血流变、肛温及耳部皮肤病理变化对模型进行评价，并采用消银片（2.1 g/kg）对其进行反证。结果 与对照组比较，模型组豚鼠出现典型的血热证银屑病症状，肛温明显升高（P<0.05、0.01），全血黏度低切（LS）、中切（MS）、高切（HS）值均显著升高（P<0.01），耳组织质量和厚度均显著增加（P<0.05、0.01）；Baker评分及炎细胞浸润数显著升高（P<0.01）；肉眼观察，模型组豚鼠耳部潮红，皮肤表面形成结痂，出现白色银屑，触之耳组织表面粗糙、质地变硬、厚度增加；镜检结果显示，模型组豚鼠皮肤角化过度形成较厚的结痂，颗粒层不完整，棘层显著增厚，表皮突下延，乳突伸长呈杵状，新生毛细血管不同程度增多且血管充血，伴有不同程度水肿及炎细胞浸润。与模型组比较，消银片组右耳厚度及质量，耳组织平均厚度及平均质量均显著下降（P<0.05）；Baker评分及炎细胞浸润数均显著降低（P<0.05）。结论 豚鼠血热证银屑病模型既有银屑病的病理特征，又具有中医血热证的证候表现，是适用于中药的疗效评价和作用机制研究的实验动物模型。

Establishment and evaluation of a compound model of psoriasis (blood heat syndrome) in guinea pigs

Objective Based on the etiology of the blood-heat syndrome of traditional Chinese medicine in psoriasis and the combination of the symptoms of psoriasis, the guinea pig composite model of psoriasis syndrome was established. Methods The method of muti-facto compound molding was adopted, Model of blood-heat Syndrome induced by ig of compound hot medicine in Guinea Pigs, Ears'' backs of guinea pigs were treated topically with 5% propranolol emulsion to create a psoriasis model,The model was evaluated by physical signs, blood rheology, anal temperature and pathological changes of ear skin. Xiaoyin tablets (2.1 g/kg) were used to prove it. Results Compared with control group, guinea pigs in model group had typical symptoms of psoriasis of blood heat syndrome, anal temperature was significantly increased (P<0.05, 0.01), whole blood viscosity low cut (LS), middle cut (MS), high cut (HS) values were significantly increased (P<0.01), ear tissue mass and thickness were significantly increased (P<0.05, 0.01); Baker score and inflammatory cell infiltration number were significantly increased (P<0.01). Naked eye observation showed that the ears of guinea pigs in the model group were flushed, scabs were formed on the surface of the skin, and white silver filings appeared on the surface of the skin. The surface of ear tissue was rough, the texture became hard and the thickness increased. The results of microscopic examination showed that in the model group, the skin hyperkeratosis formed thick scab, the granular layer was incomplete, the spinous layer was significantly thickened, the epidermal process was extended downward, the mastoid process was elongated like a pestle, the number of new capillaries increased in varying degrees and the blood vessels were congested, accompanied by edema and inflammatory cell infiltration in different degrees. Compared with the model group, the thickness and weight of right ear, average thickness and average mass of ear tissue in Xiaoyin tablet group were significantly decreased (P<0.05), Baker score and inflammatory cell infiltration number were significantly decreased (P<0.05). Conclusion The Guinea pig model of psoriasis with blood-heat syndrome has not only the pathological features of psoriasis, but also the syndrome manifestations of blood-heat syndrome, An experimental animal model for the efficacy evaluation and mechanism of traditional Chinese medicine in the treatment of psoriasis.