| 川楝子乙醇部位HPLC指纹图谱研究 |
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| 引用本文： | 周浓,杨勤,李洁玉,施晓燕,姜北.川楝子乙醇部位HPLC指纹图谱研究[J].中国现代应用药学,2012,29(9):814-818. |
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| 作者姓名： | 周浓 杨勤 李洁玉 施晓燕 姜北 |
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| 作者单位： | 1.大理学院药学院，云南 大理 671000；2.重庆三峡学院生命科学与工程学院，重庆 万州 404000;重庆三峡医药高等专科学校，重庆 万州 404020;重庆三峡医药高等专科学校，重庆 万州 404020;大理学院药学院，云南 大理 671000;大理学院药学院，云南 大理 671000 |
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| 摘 要： | 目的建立川楝子乙醇提取部位的HPLC指纹图谱。方法色谱柱Agilent zorbax SB-C18柱(4.6 mm×150 mm,5μm);流动相甲醇/乙腈(1∶10)(A)-0.4%磷酸溶液(B),梯度洗脱;检测波长360 nm;柱温25℃;流速1.0 mL.min 1。结果建立了川楝子药材乙醇提取部位的HPLC色谱指纹图谱。在指纹图谱中共有峰20个,并指认了3个色谱峰。结论该方法准确可靠,重复性好,可为川楝子药材及其饮片的质量控制提供参考依据。
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| 关 键 词： | 川楝子 乙醇部位 高效液相色谱法 指纹图谱 |
| 收稿时间： | 2011/12/1 0:00:00 |
| 修稿时间： | 2012/7/12 0:00:00 |
HPLC Fingerprints of Ethanolic Part of Toosendan Fructus |
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| ZHOU Nong,YANG Qin,LI Jieyu,SHI Xiaoyan and JIANG Bei.HPLC Fingerprints of Ethanolic Part of Toosendan Fructus[J].The Chinese Journal of Modern Applied Pharmacy,2012,29(9):814-818. |
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| Authors: | ZHOU Nong YANG Qin LI Jieyu SHI Xiaoyan and JIANG Bei |
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| Institution: | 1.College of Pharmacy, Dali University, Dali 671000, China; 2.College of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404000, China;Chongqing Three Gorges Medical College, Chongqing 404020, China;Chongqing Three Gorges Medical College, Chongqing 404020, China;College of Pharmacy, Dali University, Dali 671000, China;College of Pharmacy, Dali University, Dali 671000, China |
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| Abstract: | OBJECTIVE To establish HPLC fingerprints of ethanolic extraction part of Toosendan Fructus.METHODS Agilent zorbax SB-C18 column（4.6 mm×150 mm,5 μm） was adopted.A gradient elution was applied with mobile phase A（methanol and acetonitrile 1∶10） and mobile phase B（0.4% phosphoric acid solution）.The flow rate was 1.0 mL.min 1,the detective wavelength was 360 nm,and the column temperature was 25 ℃.RESULTS HPLC fingerprints of ethanolic extraction part of Toosendan Fructus was established.Twenty peaks were observed on the HPLC fingerprints of Toosendan Fructus and 3 of them were identified.CONCLUSION The method developed in this study is accurate with high repeatability,and available for the quality control of Toosendan Fructus and its decoction pieces. |
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| Keywords: | Toosendan Fructus ethanolic part HPLC fingerprint |
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