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HPLC波长切换法同时测定沉香舒气丸中9种成分的含量
引用本文:林源,张成中.HPLC波长切换法同时测定沉香舒气丸中9种成分的含量[J].中国现代应用药学,2020,37(5):582-586.
作者姓名:林源  张成中
作者单位:温岭市中医院, 浙江 温岭 317500,阜阳市食品药品检验检测中心, 安徽 阜阳 236015
摘    要:目的建立并验证同时测定沉香舒气丸中α-香附酮、木香烃内酯、去氢木香内酯、原阿片碱、延胡索乙素、紫堇碱、四氢小檗碱、和厚朴酚、厚朴酚含量的HPLC波长切换方法。方法采用Kromasil Eternity C18色谱柱(250 mm×4.6 mm,5μm);流动相A为甲醇-乙腈(1∶1),流动相B为0.1%磷酸溶液,梯度洗脱;检测波长分别为250,225,280,294 nm;体积流量0.9 mL·min-1;柱温30℃。结果 9种成分α-香附酮、木香烃内酯、去氢木香内酯、原阿片碱、延胡索乙素、紫堇碱、四氢小檗碱、和厚朴酚、厚朴酚分别在5.79~115.80,7.86~157.20,9.68~193.60,1.07~21.40,3.09~61.80,3.77~75.40,2.09~41.80,15.77~315.40,19.86~397.20μg·mL-1内线性关系良好;平均加样回收率分别为97.95%,98.97%,99.46%,97.37%,98.48%,98.10%,96.90%,99.35%和100.10%,RSD分别为1.35%,1.17%,0.89%,1.57%,1.27%,0.96%,1.09%,0.74%和0.90%。结论所建立的测定方法操作便捷、重复性好,可用于沉香舒气丸的质量控制。

关 键 词:沉香舒气丸  波长切换法  高效液相色谱法  化学成分
收稿时间:2019/3/19 0:00:00

Simultaneous Determination of Nine Compositions in Chenxiang Shuqi Wan by HPLC Wavelength Switching Method
LIN Yuan and ZHANG Chengzhong.Simultaneous Determination of Nine Compositions in Chenxiang Shuqi Wan by HPLC Wavelength Switching Method[J].The Chinese Journal of Modern Applied Pharmacy,2020,37(5):582-586.
Authors:LIN Yuan and ZHANG Chengzhong
Institution:Wenling Hospital of Traditional Chinese Medicine, Wenling 317500, China and Fuyang Institute for Food and Drug Control, Fuyang 236015, China
Abstract:OBJECTIVE To develop and validate an HPLC wavelength switching method for detemining contents of α-cyperone, costunolide, dehydrocostuslactone, protopine, tetrahydropalmatine, corydaline, tetrahydroberberine, honokiol and magnolol in Chenxiang Shuqi Wan. METHODS HPLC method was employed on a Kromasil Eternity C18 chromatographic column(250 mm×4.6 mm, 5 μm). The mobile phase was methanol-acetonitrile(1∶1)(A)-0.1% phosphoric acid solution(B) with gradient elution. The detection wavelengths were 250, 225, 280, 294 nm. The flow rate was 0.9 mL·min-1, and the column temperature was 30 ℃. RESULTS The liner ranges of α-cyperone, costunolide, dehydrocostuslactone, protopine, tetrahydropalmatine, corydaline, tetrahydroberberine, honokiol and magnolol were within 5.79-115.80, 7.86-157.20, 9.68-193.60, 1.07-21.40, 3.09-61.80, 3.77-75.40, 2.09-41.80, 15.77-315.40, 19.86-397.20 μg·mL-1. The average recovery rates were 97.95%, 98.97%, 99.46%, 97.37%, 98.48%, 98.10%, 96.90%, 99.35% and 100.10%, with RSD of 1.35%, 1.17%, 0.89%, 1.57%, 1.27%, 0.96%, 1.09%, 0.74% and 0.90%. CONCLUSION The method was easy to operate and repeatable, can be used for quality control of Chenxiang Shuqi Wan.
Keywords:Chenxiang Shuqi Wan  wavelength switching method  HPLC  chemical composition
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