美洲大蠊提取物Ento-A对大鼠急性胃炎胃黏膜的保护作用

目的研究美洲大蠊提取物Ento-A对急性胃炎胃黏膜的保护作用及其初步作用机制。方法将大鼠分为正常对照组,模型对照组,康复新液组,西咪替丁组,美洲大蠊提取物Ento-A高、低剂量组,连续给药8 d后,采用80%乙醇与20 mg·mL-1水杨酸钠溶液等体积混合后灌胃建立胃黏膜损伤模型,以胃炎发生计分及病理组织学评分评价Ento-A对胃黏膜的保护作用;酶联免疫法检测大鼠血清中丙二醛(MDA)、肿瘤坏死因子-α(TNF-α)、超氧化物歧化酶(SOD)、白介素-1β(IL-1β)及胃组织匀浆中表皮细胞生长因子(epidermal growth factor,EGF)的表达水平,探究Ento-A对胃黏膜的保护作用机制。结果与模型对照组比较,康复新液、西咪替丁及Ento-A能显著降低急性胃炎大鼠血清中TNF-α、MDA、IL-1β和升高SOD的表达水平(P<0.05,P<0.01),并能提高大鼠急性胃炎胃组织匀浆中EGF的表达(P<0.05,P<0.01)。结论美洲大蠊提取物Ento-A具有明显的胃黏膜损伤保护作用,其机制可能与减少炎性因子分泌,抗自由基损伤及促进组织修复有关。

Protective Effect of Ento-A Extract of Periplaneta Americana on Gastric Mucosa in Rats with Acute Gastritis

OBJECTIVE To study the protective effect of Ento-A extract from Periplaneta americana on gastric mucosa of acute gastritis and to explore its mechanism. METHODS Rats were divided into normal control group, model control group, Kangfuxin liquid group, cimetidine group, Ento-A high and low dose group. After 8 days of continuous administration, the model of gastric mucosal injury was established by intragastric administration with 20 mg·mL-1 sodium salicylate solution and 80% ethanol. The protective effect of Ento-A extract on the gastric mucosa was evaluated by gastritis scoring and histopathological scoring. The expression levels of MDA, TNF-α, SOD, IL-1β in serum and epidermal growth factor(EGF) in gastric tissue homogenate of rats were detected by ELISA to investigate the protective mechanism of Ento-A on gastric mucosa. RESULTS Compared with the model control group, Kangfuxin liquid, cimetidine and Ento-A could significantly reduce the expression of TNF-α, MDA, IL-1β, and increase the level of SOD in the serum of rats with acute gastritis(P<0.05, P<0.01), increase the expression of EGF in the gastric homogenate of rats with acute gastritis(P<0.05, P<0.01). CONCLUSION Ento-A has obvious protective effect on gastric mucosal injury, and its mechanism may be related to reducing the secretion of inflammatory factors, resisting free radical damage and promoting tissue repair.