离体培养人肠道菌群对黄山药总皂苷的代谢研究

目的通过离体实验观察人肠道内细菌对黄山药总皂苷的代谢。方法离体培养人肠道内细菌，与黄山药总皂苷厌氧温孵，薄层色谱检测黄山药总皂苷的代谢，采用硅胶柱色谱对黄山药总皂苷肠道代谢产物进行分离和代谢转化产物的结构鉴定。结果代谢产物经IR、EI—MS、DEPT和NMR鉴定为，25（R）-螺甾-5-烯-3β，20（S）-二醇，此化合物为新的天然产物。结论体外模拟的人肠道菌群对黄山药总皂苷有代谢作用，其中转化出的新成分是不是起药理活性的真正成分，有待进一步确证。

Study on metabolism of Dioscoreapan thaica Prainet Burkill in intestinal flora in vitro culture

Objective to study TSDP metabolism in human intestinal flora in vitro. Method human intestinal flora and TSDP were incubated in vitro,TLC were used to examine TSDP and its metabolites, one metabolite were separated and purified by silica gels column chromatograp-hy. Result The structure were identified by IR,M S,DEPT and NMR as 25 （R）-spirost-5-en-3β,20 （S）- diol. Conclusion Human intestinal flora in vitro have metabolism effect on TSDP,whether the metabolite has real pharmacologicl activity needs to be further investigated.