| F1013对D-Gal N/LPS诱导大鼠急性肝损伤的治疗作用 |
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| 引用本文： | 邓利娟,李湛军,罗楹,范慧红.F1013对D-Gal N/LPS诱导大鼠急性肝损伤的治疗作用[J].中国新药杂志,2011(8). |
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| 作者姓名： | 邓利娟 李湛军 罗楹 范慧红 |
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| 作者单位： | 中国药品生物制品检定所;上海睿星基因技术有限公司; |
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| 摘 要： | 目的:研究F1013对D-氨基半乳糖(D-Gal N)及脂多糖(LPS)所致大鼠急性肝损伤模型的治疗作用,并对其机制进行初步探讨。方法:60只雄性W istar大鼠随机分成对照组、模型组、阳性药组(N-乙酰半胱氨酸,155 m·gkg-1)和F1013给药组(5,2.5,1.25 m·gkg-1)。除对照组外,各组大鼠均腹腔注射D-GalN/LPS建立大鼠急性肝损伤模型,造模后2 h,阳性药组和F1013给药组分别腹腔注射NAC和F1013,其余组腹腔注射等体积生理盐水。造模后10 h,留取血清和肝组织,用全自动生化分析仪检测血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)和总胆红素(T-Bil)含量;用苏木素-伊红(HE)染色,观察肝组织病理学变化;采用流式细胞学技术和原位末端标记(TUNEL)法检测肝细胞凋亡情况。结果:在D-GalN/LPS诱导大鼠急性肝损伤模型,各剂量F1013均明显改善肝脏病理组织损伤;明显降低血清ALT,AST,T-Bil水平及肝细胞凋亡率(P<0.05)。结论:F1013对D-GalN/LPS所致大鼠急性肝损伤具有较好的治疗作用,其机制可能与抑制肝细胞异常凋亡有关。
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| 关 键 词： | F1013 急性肝损伤 凋亡 |
Therapeutic effect of F1013 on D-Gal N/LPS-induced acute hepatic injury in rats |
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| DENG Li-juan,LI Zhan-jun,LUO Ying,FAN Hui-hong.Therapeutic effect of F1013 on D-Gal N/LPS-induced acute hepatic injury in rats[J].Chinese Journal of New Drugs,2011(8). |
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| Authors: | DENG Li-juan LI Zhan-jun LUO Ying FAN Hui-hong |
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| Institution: | DENG Li-juan1,LI Zhan-jun1,LUO Ying2,FAN Hui-hong1(1 National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050,China,2 Shanghai Genomics Inc.,Shanghai 201203,China) |
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| Abstract: | Objective:To investigate the therapeutic effect of F1013 on D-Gal N/LPS-induced acute hepatic injury(AHI) in rats,and its mechanism of action.Methods: 60 male Wistar rats were randomly divided into groups,and were intraperitoneally(ip) injected with D-Gal N/LPS to induce AHI.2 hours later,N-acetylcysteine(NAC),F1013 or saline was ip injected.10 hours after Gal N/LPS injection,a liver segment and blood were obtained.The contents of alanine aminotransferase(ALT),aspartate aminotransferase(AST) and total bilir... |
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| Keywords: | F1013 acute hepatic injury apoptosis |
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