| RP-HPLC法测定人血清氟康唑浓度及其临床应用 |
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| 引用本文： | 盛晓燕,熊歆,杨文领,段京莉.RP-HPLC法测定人血清氟康唑浓度及其临床应用[J].中国新药杂志,2010(9). |
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| 作者姓名： | 盛晓燕 熊歆 杨文领 段京莉 |
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| 作者单位： | 北京大学第三医院1药剂科2肾内科; |
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| 摘 要： | 目的:建立反相高效液相色谱法(RP-HPLC)以测定人血清中氟康唑浓度,并用于临床1例氟康唑引起肝损害患者的药物浓度测定。方法:色谱柱为XDB C18柱(150 mm×4.6 mm,5μm),流动相:0.01mol.L-1磷酸二氢钾(pH7.4)-乙腈-甲醇(74∶16∶10),流速:1.0 mL.min-1,检测波长:210 nm;内标:氯丙那林。结果:血清氟康唑浓度在0.2~20μg.mL-1内线性良好,回归方程Y=0.188 4X+0.010 4(n=7,r=0.999 8),日内精密度RSD<5.36%,日间精密度RSD<10.83%,方法提取回收率为74.15%~80.68%。结论:本测定方法快速、灵敏,适用于氟康唑的血清浓度测定。关键词]氟康唑;反相高效液相色谱法;血药浓度
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| 关 键 词： | 氟康唑 反相高效液相色谱法 血药浓度 |
Determination of fluconazole concentration in human serum by RP-HPLC and its clinical application |
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| SHENG Xiao-yan,XIONG Xin,YANG Wen-ling,DUAN Jing-li.Determination of fluconazole concentration in human serum by RP-HPLC and its clinical application[J].Chinese Journal of New Drugs,2010(9). |
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| Authors: | SHENG Xiao-yan XIONG Xin YANG Wen-ling DUAN Jing-li |
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| Institution: | SHENG Xiao-yan1,XIONG Xin1,YANG Wen-ling2,DUAN Jing-li1(1 Department of Pharmacy,2 Department of Nephrology,Peking University Third Hospital,BeiJing 100191,China) |
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| Abstract: | |
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| Keywords: | fluconazole RP-HPLC plasma concentration |
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