二苯乙烯苷对脑缺血再灌注沙土鼠学习记忆功能及NMDA受体亲合力的影响

目的:研究2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside,简称二苯乙烯苷)对脑缺血再灌注沙土鼠学习记忆功能和对N-甲基-D-天冬氨酸(NMDA)受体亲合力的影响.方法:夹闭沙土鼠双侧颈总动脉10min,然后撤去动脉夹实现再灌注,7d后通过水迷宫测定学习记忆功能的改变,随后处死,测定前脑组织NMDA受体与3H]MK801的亲合力.结果:Morris水迷宫实验显示,模型组游出时间和游泳距离比假手术组明显延长,二苯乙烯苷治疗组的游出时间和游泳距离均比模型组缩短.受体亲合情况测定结果显示,模型组的NMDA受体与3H]MK801的亲和力比假手术组明显升高,而二苯乙烯苷治疗组可以降低模型动物的NMDA受体亲合力.结论:二苯乙烯苷能够改善缺血再灌注所导致的学习记忆功能障碍,降低NMDA受体与3H]MK801亲合力,可能具有脑保护作用.

The effects of tetrahydroxylstilbene on learning and memory ability and NMDA-receptor binding to [3H]MK801 inforebrain of ischemia-reperfusion gerbils

Objective:To study the effects of tetrahydroxylstilbene on learning and memory a-bility and NMDA (N-methyl-D-aspartate) receptor to 3H]MK801 in brain of ischemia-reperfusion gerbils. Methods:The bilateral carotid ateries of gerbils were occluded for 10min with micro-aneurysm clips and then reperfused. 7d later, the alternation in learning and memory ability was evaluated by MWM (Morris water maze). NMDA receptor binding force of forebrain tissue was assayed after sacrifice of gerbils. Results: Result of MWM evaluation showed that the swimming time and distance of model group were significantly prolonged compared with sham-operation group, while those of tetrahydroxylstilbene treated group were shortened. The assay of NMDA receptor binding test indicated that the receptor binding force of model group was higher than that of sham-operation group. Tetrahydroxylstilbene treatment can markedly reduce the binding force. Conclusion: Tetrahydroxylstilbene can ameliorate the learning and memory dysfunction of ischemia-reperfusion gerbil model and reduce NMDA receptor-binding force,so tetrahydroxylstilbene may have brain protective effect.