Attenuating effects of Granulocyte-colony stimulating factor (G-CSF) in radiation induced intestinal injury in mice |
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Authors: | Joong-Sun Kim Seung-Bum Ryoo Kyu Heo Joong-Gook Kim Tae-Gen Son Changjong Moon Kwangmo Yang |
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Institution: | 1. Research Center, Dongnam Institute of Radiological & Medical Sciences (DIRAMS), Busan 619-953, Republic of Korea;2. Department of Surgery, Dongnam Institute of Radiological & Medical Sciences (DIRAMS), Busan 619-953, Republic of Korea;3. Department of Radiation Oncology, Dongnam Institute of Radiological & Medical Sciences (DIRAMS), Busan 619-953, Republic of Korea;4. Department of Veterinary Anatomy, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Republic of Korea;5. Department of Radiation Oncology, Korea Institute of Radiological & Medical Sciences, Seoul 139-706, Republic of Korea |
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Abstract: | Gastrointestinal injury is a major cause of death following exposure to high levels of radiation, and no effective treatments are currently available. In this study, we examined the capacity of granulocyte colony-stimulating factor (G-CSF) to mitigate intestinal injury in, and improve survival of, C3H/HeN mice given a lethal dose (12 Gy) of radiation to the abdomen. G-CSF (100 μg/kg body weight) was injected subcutaneously daily for 3 days after irradiation and shown to improve survival and intestinal morphology at 3.5 days compared with saline-injected controls. The morphological features improved by G-CSF included crypt number and depth, villous length, and the length of basal lamina of 10 enterocytes. G-CSF also normalized the levels of circulating tumor necrosis factor alpha and attenuated the loss of peripheral neutrophils, caused by radiation-induced myelosuppression. In conclusion, our results suggest that G-CSF enhanced the survival of irradiated mice and minimized the effects of radiation on gastrointestinal injury. |
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Keywords: | G-CSF Radiation Intestinal injury Survival rate Crypt |
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