汉黄芩素上调小鼠骨髓Lin-细胞Pecam1基因表达

目的]探讨汉黄芩素对小鼠骨髓Lin-细胞增殖、分化、迁移、黏附关键功能基因表达的影响。方法]采用免疫磁珠法分离小鼠骨髓Lin-细胞,采用CD117作为标志进行细胞鉴定。应用实时定量聚合酶链式反应(RT-PCR)芯片技术研究汉黄芩素对小鼠骨髓Lin-细胞功能基因表达的影响。采用黏附能力测定实验观察Lin-细胞的黏附能力。结果]流式细胞技术结果显示,小鼠骨髓源性单个核细胞免疫磁珠分选后CD117的阳性率为93.7%;芯片结果发现汉黄芩素明显上调Lin-细胞血小板内皮细胞黏附分子-1(Pecam1)基因表达;黏附能力测定实验结果可见汉黄芩素增强Lin-细胞的黏附能力。结论]免疫磁珠法分离小鼠骨髓Lin-细胞是一种较为理想的分离方法;汉黄芩素可能通过上调小鼠骨髓Lin-细胞Pecam1基因表达,从而增加Lin-细胞的黏附能力。

Regulated Pecam1 gene expression in bone marrow-derived Lin- cells by wogonin

Objective] To explore the effects of wogonin on the proliferation, differentiation, migration, adhesion gene expression of bone Marrow-derived Lin^- cells. Method] Using magnetic activated cell sorting (MACS) to separate Lin^- cells from bone marrow and using CD117 as a marker for cell identification. To investigate the effect of wogonin on functional gene expression of bone marrow-derived Lin^- cells by real-time-RT-PCR array technology. Adhesion method was used to detect the effect of wogonin on the adhesion of Lin^- cells. Result] Flow cytometry results showed that, mononuclear cells derived from bone marrow of mice after MACS, the separation rate of CD117 was 93.7%. The PCR chip results showed that the gene expression of platelet endothelial cell adhesion molecule -1 (Pecam1) of Lin-cell was significantly up-regulation by wogonin. The adhesion ability experimental result showed that wogonin could enhance the adhesion ability of Lin^- cells. Conclusion] It is an ideal separation method by using MACS to separate Lin^- cells from bone marrow. Wogonin may up regulate Lin^- cells in bone marrow of mice Pecam1 gene expression, thereby increasing the adhesion ability of Lin^- cells.