莫哈韦丝兰化学成分的研究

目的] 对莫哈韦丝兰的化学成分进行系统研究。方法] 利用正相硅胶、反相ODS、Sephadex LH-20及高效液相色谱等多种色谱法进行分离纯化，并通过理化性质及波谱分析鉴定化合物结构。结果] 从莫哈韦丝兰70%乙醇提取物中分离鉴定了14个单体化合物，分别为咖啡酸（1）、β-羟基-3-甲氧基-4-羟基苯乙酮（2）、1-O-β-D-glu-copyranosyl-2-hydroxy-4-allylbenzene （3）、2-O-（4-hydroxybenzoyl）-2，4，6-trihydroxyphenylacetic acid （4）、对羟基苯甲酸（5）、原儿茶酸（6）、4-羟基-3-甲氧基苯甲酸（7）、丁香酸（8）、对羟基苯甲醛（9）、原儿茶醛（10）、麦芽酚（11）、5-hydroxymalto （l 12）、5-羟甲基糠醛（13）和4，4-二甲基-1，7-庚二酸（14）。结论] 化合物2~4、7~14为首次从丝兰属植物中分离得到的化合物，5、6为首次从该种植物中分离得到的化合物。

Study on the constituents of Yucca schidigera Roezl (Mojave)

Objective] To observe and explore the effect of abdominal massage therapy on cerebral neurotransmitter and Orexin-A in sleep deprivation rats.Methods] The 30 Wistar rats with 10 in each group were randomly divided into normal control group,model control group,and abdominal massage therapy group. Adopting a modified multi-platform water environment method for sleep deprivation in the latter two groups of rats,Rats in the normal control group and model control group did not interfere after modeling. The rats in the abdominal massage therapy group were given a daily abdominal massage therapy. After 14 days,compare the levels of 5-HT,DA,NE,GABA,and Orexin-A in the brains of rats in each group.Results] Compared with normal control group,the levels of 5-HT,DA,NE,GABA,and Orexin-A in the brain of the model control group were significantly changed (P<0.05). Compared with the model control group,the level of 5-HT in the brain of the rats in the abdominal massage therapy group was significantly increased,and the levels of DA,NE,and Orexin-A significantly decreased (P<0.05),while there was no significant difference in GABA levels (P>0.05).Conclusion] Abdominal massage therapy can regulate the levels of 5-HT,DA,NE,and Orexin-A in the brain of rats and play a role in regulating sleep and wakefulness in animals.