血必净注射液对脓毒症大鼠器官功能及死亡率的影响*

目的]观察血必净注射液对脓毒症大鼠多器官功能及死亡率的影响。方法]采用大鼠盲肠结扎穿孔（CLP）模型。雄性Wistar大鼠96只按随机数字表法分为正常对照组（n=8）、假手术组（n=8）、模型对照组（n=40）,血必净治疗组（n=40）,后两组按观察时间点分为2、8、24、48和72 h亚组。另设实验观察血必净注射液对脓毒症大鼠的治疗效果,观察CLP术后7 d存活率。结果]血必净治疗组血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、尿素氮（BUN）、肌酐（Cr）、肌酸激酶（CK）及心肌型肌酸激酶同功酶（CK-MB）水平呈不同程度降低;与模型未治疗组比较,血必净治疗组及联合治疗组动物死亡率显著降低。结论]血必净注射液能够明显降低脓毒症大鼠的死亡率,并对重要器官功能具有保护作用。

Effects of xuebijing injection on organ function and mortality in rats with sepsis

Objective] To investigate the effects of Xuebijing (cleaning the blood) injection on them ultiple organ function and mortality in ratswith sepsis.Methods] Sepsis was reproduced in ratswith cecum ligation and puncture (CLP).Ninety-six male W istarratswererandom lydivided intonorm alcontrolgroup (n=8),sham-operation group (n=8),CLP model group (n=40),Xuebijing-treated group (n=40).The two latter groups were further divided into five groups of 2,8,24,48,and 72-hour subgroups with 8 ratsin each.In addition,another experiments were performed to observe the effect of treatment of Xuebijing on the survivalrate.Results] Serum levels of ALT,AST,blood urea nitrogen (BUN),creatinine (Cr),creatine kinase (CK) and MB isoenzyme ofcreatine kinase (CK-MB) were significantly increased with certain extentat different intervals.Early treatment with Xuebijing could markedly improved serum biochemical param eterspartly.Treatm entwith Xuebijing or/and antibiotic could significantly improve the survivalrates in septic rats.Conclusion] These finding sindicates that Xuebijing injection can obviously decline the mortality of septicrats,and hasplayed an important role in protecting against the multiple organ dysfunction.