石柱和抚松产人参叶指纹图谱分析

[目的]建立石柱和抚松人参叶药材的高效液相色谱(HPLC)指纹图谱,以探讨两产地人参叶皂苷含量的差异。[方法]采用HPLC法分别测定采于辽宁宽甸石柱、吉林抚松的人参叶HPLC指纹图谱,运用中药色谱指纹图谱相似度评价系统对各样品进行分析。[结果]10批人参叶样品的HPLC指纹图谱相似度均大于0.9,标定了22个共有峰,不同产地人参叶共有峰相对含量存在一定的差异。[结论]建立的人参叶HPLC指纹图谱分析方法科学可靠,可为人参叶药材质量研究提供参考。

Analysis on HPLC fingerprint of ginseng leaffrom Shizhuand Fusong

[Objective] To study the difference of ginsenoside content from different production places by establishing HPLC fingerprints of ginseng leaf.[Methods] HPLC fingerprints of ginseng leaf samples from Liaoning Province,Dandong City,Kuandian Manchu Autonomous County Township Shizhu Village and Jilin Province,Fusong County were analyzed by HPLC.The similarity of each sample was analyzed by similarity evaluation system for chromatographic fingerprint of chinese materia media.[Results] The chromatographic fingerprint similarity of ten batches of ginseng leaf samples is more than 0.9 and there are altogether 22 common peaks,but has some differences between the relative area of the common peaks.[Conclusion] HPLC fingerprints of ginseng leaf from different production places will provide a reference standard for the study of ginseng leaf from different production places.