| 人子宫内膜腺上皮细胞的体外分离培养及纯化 |
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| 引用本文： | 王月玲,李沛霖,李洁,王建玲,华丹丹,王倩男,王璀.人子宫内膜腺上皮细胞的体外分离培养及纯化[J].天津中医药大学学报,2017,36(4):291-294. |
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| 作者姓名： | 王月玲 李沛霖 李洁 王建玲 华丹丹 王倩男 王璀 |
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| 作者单位： | 天津中医药大学, 天津 300193,天津中医药大学第二附属医院, 天津 300150,天津中医药大学第二附属医院, 天津 300150,天津中医药大学第二附属医院, 天津 300150,天津中医药大学, 天津 300193,天津中医药大学, 天津 300193,天津中医药大学, 天津 300193 |
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| 基金项目： | 国家自然科学基金项目（81072837）。 |
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| 摘 要： | 目的]优化子宫内膜腺上皮细胞的分离培养及纯化技术,以获得经济、数量多、活性好、纯度高的子宫内膜腺上皮细胞,为子宫内膜异位症的研究提供可靠的体外模型。方法]采用复合酶重复消化、80目筛网单次过滤、低速离心、差时贴壁法处理。结果]经诊断性刮宫获取的19例标本中,18例腺上皮细胞培养成活,1例失败;腺上皮细胞纯度高达94.2%。结论]复合酶多次消化、80目筛网单次过滤、低速离心、差时贴壁法培养子宫内膜腺上皮细胞,可以获得经济、数量多、活性好、纯度高的细胞。
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| 关 键 词： | 子宫内膜异位症 腺上皮细胞 细胞培养 |
| 收稿时间： | 2017/3/16 0:00:00 |
Isolation, culture and purification of human endometrial glandular epithelial cells in vitro |
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| WANG Yue-ling,LI Pei-lin,LI Jie,WANG Jian-ling,HUA Dan-dan,WANG Qian-nan and WANG Cui.Isolation, culture and purification of human endometrial glandular epithelial cells in vitro[J].Journal of Tianjin University of Traditonal Chinese Medicine,2017,36(4):291-294. |
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| Authors: | WANG Yue-ling LI Pei-lin LI Jie WANG Jian-ling HUA Dan-dan WANG Qian-nan and WANG Cui |
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| Institution: | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China,The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China,The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China,The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China,Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China,Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China and Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract: | |
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| Keywords: | endometriosis endometrial glandular epithelial cells cells culture |
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