纳米金标记法在DNA检测及基因芯片技术中的应用

近年来DNA介导的纳米金颗粒的组装研究受到广过重视，烷疏基修饰的寡核苷酸可与纳米金共价结合，形成的探针可与目的多核苷酸序列杂交，纳米金颗粒被可逆组装成超分子结构，聚集过程伴有红色到蓝色的颜色变化，由此衍生出比色法检测特定DNA序列的新方法。该方法具有极高的选择性和较好的灵敏度，与银染法结合更提高了检测的灵敏度，可用于斑点亲交、芯片检列等领域。本文对该方法的原理、操作过程及研究进展作一简要介绍。

Applications of Au Nanoparticles Labeling Method in DNA-detection and the Technology of Genechip

Recently, the research in the DNA mediated assembling of Au nanopartic les has caused wide attention. Mercaptoalkyloligonucleotides c an be covalently bound to Au nanoparticles, which form probes. When these probes were hybridized with th e target polynucleotides sequence, the Au particles are assembled reversibly and form a supermolecular structure. The polymerization process is accompanied by a red to blue color change, providing a novel colorimetric detection technique of sequence specific DNA. This is a highly selective and sensitive method, and th e sensitivity of the detection can be further enhanced by the silver deposition. It is believed that the combination of Au nanoparticles labeling with silver dep osition amplification will find its use in the field of blot hybridization and a rray detection. This article introduced the principle,the process of the operation a nd the research progress of the technique.