人胃癌裸鼠原位移植模型的7T MRI检测

通过胶黏合法建立人胃癌裸鼠原位移植模型，并使用7T核磁共振成像(7T MRI)动态检测原位肿瘤。将对数生长期人胃低分化腺癌SGC-7901细胞制成细胞悬液注射于裸鼠右腋皮下，建立皮下移植模型；以皮下移植瘤块为材料，采用胶黏合法建立原位移植模型。造模约20 d后，裸鼠进行7T MRI扫描，并对胃部组织进行组织病理学检查。3只裸鼠中2只胃部可见肿瘤疑似物，组织病理学检查验证该疑似物为肿瘤，成瘤率达66.7%。实验结果表明，7T MRI对原位肿瘤可进行无创、动态的活体检测，且无辐射伤害。MRI检测有望用于临床前胃癌药物的研发以及临床胃癌的诊断。

MRI detection method for human gastric cancer orthotopic transplantation model in nude mice

The study aims to establish a human gastric cancer orthotopic transplantation model in nude mice and to use 7T MRI for detection. After poorly differentiated human gastric adenocarcinoma SGC-7901 cells were injected subcutaneosly into the right flanks of nude mice, the model of in nude mice was established with orthotopic transplanted cancer of gastric tumor by the Compont^® gel pasted method. 7T MRI scan was conducted on the mice after operating model about 20 days later. Histopathological examinations were carried out on the stomach. Two of three mice on which 7T MRI scan were performed showed visible suspected stomach tumor and their presence was verified again by histopathological examinations; tumor formation rate in the nude mice gastric orthotopic transplantation model was 66. 7%. This study suggested that 7T MRI could be used in the live detection of in situ tumor and that MRI could be used for pre-clinical gastric cancer drug development and clinical gastric carcinoma diagnosis.