首页 | 本学科首页   官方微博 | 高级检索  
     

大鼠灌服枳实提取液后体内黄酮类代谢产物的LC-MS/MS分析
引用本文:李 哲, 宋 瑞, 许风国, 许 磊, 田 媛, 张尊建. 大鼠灌服枳实提取液后体内黄酮类代谢产物的LC-MS/MS分析[J]. 中国药科大学学报, 2010, 41(6): 539-547.
作者姓名:李 哲  宋 瑞  许风国  许 磊  田 媛  张尊建
作者单位:1.中国药科大学药物质量与安全预警教育部重点实验室;2.中国药科大学分析测试中心
基金项目:国家自然科学基金资助项目(No.30672587)
摘    要:建立了高效液相色谱-串联质谱分析方法,分析大鼠灌服枳实提取液后尿液、粪便和胆汁中的黄酮类成分。采用色谱柱为Agilent Zorbax SB-C 18柱(250 mm×4.6 mm,5 μm);流动相为0.1%甲酸水溶液-甲醇-乙腈三元梯度洗脱系统,柱温35 ℃,流速1.0 mL/min;采用电喷雾离子化源-三重四极杆质谱系统,负离子模式检测。在枳实提取液中共检出了34种黄酮类成分,在大鼠生物样本中共检出40种黄酮类成分,其中14种来源于体外,26种为代谢产物,并初步归属了它们的结构。

关 键 词:枳实  黄酮  代谢产物  体内代谢  高效液相谱-串联质谱法

Identification of flavonoid metabolites of Fructus Aurantii Immaturus in rat biological samples by liquid chromatography-tandem mass spectrometry
LI Zhe, SONG Rui, XU Feng-guo, XU Lei, TIAN Yuan, ZHANG Zun-jian. Identification of flavonoid metabolites of Fructus Aurantii Immaturus in rat biological samples by liquid chromatography-tandem mass spectrometry[J]. Journal of China Pharmaceutical University, 2010, 41(6): 539-547.
Authors:LI Zhe  SONG Rui  XU Feng-guo  XU Lei  TIAN Yuan  ZHANG Zun-jian
Abstract:High-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) was used for separation and identification of flavonoid metabolites in rat urine,feces and bile after oral administration of the Citrus sinensis extraction.The HPLC experiments used a reversed-phase Zorbax SB-C 18 column with the column temperature of 35 °C and a binary mobile phase system consisting of aqueous formic acid (0.1%) and methanol-acetonitrile (3∶2) using a gradient elution with the flow rate of 1.0 mL/min.The ESI-MS/MS was operated with a triple-quadruple mass spectrometer in negative ion mode.A total of 34 flavonoid compounds in vitro and 40 flavonoid compounds including 14 parent compounds and 26 metabolites in vivo were detected,and they were tentatively identified.The identification of these metabolites provided essential data for further pharmacological and clinical studies of Citrus sinensis Osbeck.and related preparations.
Keywords:Citrus sinensis Osbeck.  flavonoid  metabolites  in vivo metabolism  LC-MS/MS
点击此处可从《中国药科大学学报》浏览原始摘要信息
点击此处可从《中国药科大学学报》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号