基于肝微粒体和指纹图谱研究六味地黄丸与硝苯地平的药物相互作用

本研究建立了基于体外肝微粒体和中药代谢指纹图谱研究六味地黄丸与硝苯地平的药物相互作用的方法。体外肝微粒体温孵采用加入六味地黄丸和硝苯地平置37 ℃水浴中温孵60 min。指纹图谱测定采用C₁₈色谱柱(4.6 mm×250 mm，5 μm)，以乙腈-0.1%甲酸为流动相梯度洗脱，流速1.0 mL/min，检测波长240 nm；测定温孵前后微粒体中六味地黄丸的代谢指纹图谱，通过相似度比较评价两者的相互作用。结果显示六味地黄丸与硝苯地平共孵前后，代谢指纹图谱无明显差异。在体动物试验显示，六味地黄丸与硝苯地平联合给药前后，典型成分的主要药代动力学参数无显著差异。

Investigation of herb-drug interaction between Liuwei Dihuang Pills and nifedipine based on rat liver microsomes and HPLC fingerprints

A new method based on rat liver microsomes and chromatographic fingerprint comparison was established to investigate the possible herb-drug interactions between Liuwei Dihuang Pills(LDP)and nifedipine(NIF). LDP and NIF were incubated simultaneously with rat liver microsomes at 37 °C for 60 min in a shaking water bath. The separation was achieved on a C₁₈ column using 0. 1% formic acid solution and acetonitrile as mobile phase with a liner gradient program. The flow rate was set at 1. 0 mL/min. Detection was achieved by UV light at 240 nm. To evaluate the interactions between LDP and NIF, the similarity of the fingerprinting chromatograms before and after co-incubation was calculated by similarity evaluation system for chromatographic fingerprint of TCM. Furthermore, pharmacokinetic experiments in rat suggested that there was no significant difference in the pharmacokinetic parameters of the typical components in LDP.