RP—HPLC法测定穿龙薯蓣总皂甙中薯蓣皂甙元的含量

目的：建立反相高效液相色谱法8测定穿龙薯蓣总皂甙中薯皂甙元的含量：方法：采用uBOndapak C18柱，甲醇为流动相，流速1．0ml/min,996二极管阵列检测器（DAD），检测波长为208nm，对穿龙薯蓣总皂甙中薯蓣皂甙元含量进行测定。结果：能将薯蓣皂甙元与其主要杂质分离开来，薯蓣皂甙元在1．90－9．51ug范围内峰面积与其浓度线性关系良好（r=0.9985)，样品平均回收率为96．15％，精密度RSD为1．72％（n=5)，结论：该法测定样品无需要衍生化，分离效果好，分析快速准确。

RP-HPLC Determination of Diosgenin in Total Saponin from Dioscorea Nipponica Mak

AIM: To develop the method of the determination of total saponinsin Dioscorea nipponica Mak. by reversed-phase high performance liquid chromatography. METHOD: This method used μ Bondapak C18 column with methanol as mobile phase, 1.0 ml/min as flow and 996-photodiode array detector at 208 nm. RESULTS: It can separate diosgenin and its primary impurity. The calibration curve was linear (r=0.998 5) in the range of 1.90～9.51 μg for diosgenin. The average recovery and precision (RSD) for diosgenin were 96.15%(n=5) and 1.72%(n=5) respectively. CONCLUSION: Using the method derivatization is not needed in determining sample and the result of separation is good. The analysis is accurate, and satisfactory.