宫颈病灶HSV-2感染及其与宫颈癌的关联性

目的观察宫颈病灶中单纯疱疹病毒-2（HSV-2）感染状况，探讨宫颈HSV-2感染与宫颈癌的关联性。方法101例宫颈病变患者中宫颈癌患者46例（癌变组），宫颈良性疾病55例（对照组），分别对其宫颈病灶组织和宫颈分泌物进行HSV-2DNA（实时荧光PCR法）和人乳头瘤病毒（HPV）基因分型（快速导流杂交法）检测。结果宫颈病灶HSV-2DNA阳性率为10．89％；其中癌变组HSV-2的阳性率为17．39％，对照组为5．45％，两组间差异无统计学意义（P〉0．05）。宫颈病灶HPV总阳性率为67．33％，共检测出13种HPV亚型，以16型最多见（45．45％）；癌变组（97．83％）与对照组（41．82％）间比较差异有统计学意义（χ^2=34．087，P〈0．001）。HSV-2与HPV合并阳性率为9．90％，HSV-2感染的癌变组中HPV阳性率达100％，混合感染的亚型主要为HPV16占75％。结论HSV-2多与HPV16亚型合并感染宫颈癌患者，其在宫颈癌演变过程中的潜在影响不能忽视。

Herpes simplex virus-2 （HSV-2） infection in cervical lesions and the relationship between cervical HSV-2 infection and cervical cancer

Objective To observe HSV-2 infection status in cervical lesions, and explore the relationship between cervical HSV-2 infection and cervical cancer. Methods Cervical lesions and cervical secretions were collected from 101 patients with cervical lesion, HSV-2 DNA （real time PCR method） and human papilloma virus （HPV） genotype （flow-through hybridization） were detected. Results The positive rate of HSV-2 DNA in cervical lesions was 10.89%, and that in cancer group and control group was 17.39% and 5.45%, respectively, with no significant difference between the two groups. The positive rate of HPV test in cervical lesions was 67.33%, and 13 subtypes of HPV were found in cervical lesions. The positive rate of the most common subtype of type 16 was 45.45%, and that in cancer group and control group was 97.83% and 41.82%, respectively, with the statistically significant difference between the two groups. （χ^2=34.087, P〈0.001）. The positive rate of HSV-2 merged with HPV was 9.90%. The positive rate of HPV in cancer group of HSV-2 infection was 100%. mixed infections of HPV16 subtype accounted for 75 %. Conclusion HSV-2 is more common in co-infected with HPV16 subtype in patients with cervical cancer, and the potential impact of HSV-2 on cervical evolution should not be ignored.