SNS-032对紫杉醇耐药乳腺癌细胞生长的抑制作用研究

目的：研究细胞周期蛋白依赖性激酶抑制剂SNS-032对人紫杉醇耐药MCF-7/TAX乳腺癌细胞生长的作用。方法采用MTT试验检测MCF-7/TAX细胞对紫杉醇的耐药情况以及SNS-032对紫杉醇耐药MCF-7/TAX细胞生长的影响；采用Annexin V-FITC/PI双染试验检测SNS-032对MCF-7/TAX耐药细胞凋亡的诱导作用。结果 MCF-7/TAX细胞对紫杉醇耐药指数达19.39；而SNS-032能显著抑制MCF-7/TAX耐药细胞的增殖，且具有明显的浓度依赖性，对其作用48 h的IC50为164.5 nmol/L，MCF-7/TAX对SNS-032的耐药指数仅为0.89。SNS-032作用后，Annexin V染色阳性的MCF-7/TAX细胞比例较对照明显升高，差异具有统计学意义(P<0.05)。结论 SNS-032能激活MCF-7/TAX耐药细胞凋亡的发生，从而对紫杉醇耐药MCF-7/TAX细胞的生长发挥高效抑制作用。

Anti-proliferative effect of SNS-032 on taxol-resistant breast cancer cells

Objective To investigate the effect of the cyclin-dependent kinase inhibitor SNS-032 on the growth of taxol-resistant MCF-7/TAX breast cancer cells. Methods The resistance situation of MCF-7/TAX cells to paclitaxel and the effect of SNS-032 on the growth of taxol-resistant MCF-7/TAX cells were evaluated by MTT (3-4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) assay. The effect of SNS-032 on apoptosis in MCF-7/TAX cells was analyzed by Annexin V-FITC/PI staining. Results The resistance index of MCF-7/TAX cells to paclitaxel was 19.39. After treatment with SNS-032 for 48 h, MCF-7/TAX cells displayed markedly inhibited growth in a dose-de-pendent manner, and calculated IC50 was 164.5 nmol/L. SNS-032 decreased the resistance index of MCF-7/TAX from 19.39 to 0.89. The numbers of apoptotic MCF-7/TAX cells, as revealed by Annexin V binding, significantly increased upon SNS-032 treatment (P<0.05). Conclusion The results demonstrate that SNS-032 can induce apoptosis in MCF-7/TAX cells and consequently display potent cytocidal effect on taxol-resistant MCF-7/TAX breast cancer cells.