| 氧化应激反应对肥胖型哮喘小鼠气道炎症和重建影响 |
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| 引用本文： | 陈凯,韩伟,唐华平,苏毅.氧化应激反应对肥胖型哮喘小鼠气道炎症和重建影响[J].齐鲁医学杂志,2011,26(6):510-512,514. |
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| 作者姓名： | 陈凯 韩伟 唐华平 苏毅 |
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| 作者单位： | 青岛市市立医院东院呼吸科,山东青岛,266071 |
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| 基金项目： | 青岛市科技发展指导计划 |
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| 摘 要： | 目的探讨氧化应激反应在肥胖型哮喘小鼠体内变化及其与气道炎症和重建的关系。方法 45只雌性C57/6J小鼠随机分为哮喘组(A组)、肥胖哮喘组(B组)和对照组(C组)。卵清蛋白雾化吸入和高脂饮食诱导建立肥胖型哮喘模型。计数各组小鼠支气管肺泡灌洗液(BALF)中白细胞总数并分类,肺组织切片观察病理变化,并测定支气管壁总面积(WAt)、支气管平滑肌面积(WAm)、管腔基底膜周长(Pbm),ELISA法测定肺组织匀浆中白细胞介素6(IL-6)和8-异前列腺素2α(8-iso-PGF2α)水平。结果 B组小鼠BALF中白细胞总数和嗜酸性粒细胞比例、肺组织IL-6水平以及肺组织切片中WAt/Pbm和WAm/Pbm均较A组明显增高(F=2.84~32.67,P<0.05)。C、A、B组小鼠血清8-iso-PGF2α水平依次升高,且与BALF中白细胞总数、肺组织IL-6水平以及WAt/Pbm存在正相关性(r=0.821、0.900、0.873,P<0.01)。结论氧化应激反应参与了肥胖型哮喘小鼠气道炎症和重建过程,抑制氧化应激可望成为肥胖型哮喘治疗的有效措施。
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| 关 键 词： | 哮喘 肥胖症 氧化性应激 小鼠 |
EFFECTS OF OXIDATIVE STRESS ON AIRWAY INFLAMMATION AND RECONSTRUCTION IN OBESE MICE WITH ASTHMA |
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| CHEN Kai,HAN Wei,TANG Huaping,SU Yi.EFFECTS OF OXIDATIVE STRESS ON AIRWAY INFLAMMATION AND RECONSTRUCTION IN OBESE MICE WITH ASTHMA[J].Medical Journal of Qilu,2011,26(6):510-512,514. |
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| Authors: | CHEN Kai HAN Wei TANG Huaping SU Yi |
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| Institution: | CHEN Kai,HAN Wei,TANG Huaping,SU Yi(Department of Respirology,Qingdao Municipal Hospital,Qingdao 266071,China) |
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| Abstract: | Objective To study oxidative stress reaction in obese mice with asthma and its association with airway inflammation and reconstruction.Methods This study consisted of 45 female C57BL/6J mice,which were randomized to three groups as group A(asthma group),group B(obese asthma group) and group C(control group).An obese asthma model was created by egg-albumin aerosol rebreathing method and high fat diet.Total white blood cells(WBC) and classification in BALF were counted;a lung tissue section was observed patho... |
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| Keywords: | asthma obesity oxidative stress mice |
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