rhEPO结合高低氧对贫血荷瘤小鼠放射增敏的效应

①目的 观察促红细胞生成素（rhEPO)结合高低氧对贫血荷瘤小鼠放射增敏的效应。②方法 昆明小鼠随机分为3组：A组加用rhEPO预防卡铂诱导的贫血，然后对小鼠荷瘤肢行高低氧放疗；B组用卡铂诱导小鼠贫血，对小鼠荷瘤肢行高低氧放疗；C组用卡铂诱导小鼠贫血，对小鼠荷瘤肢放疗。观察3组放射效应的差别，最后各组均行肿瘤组织活检。③结果 从肿瘤种植后第14天开始，A组肿瘤重新生长的速度与B，C组比较明显延缓（T＝0－3，P＜0.05)；C组与B组比较，B组肿瘤细胞种植后第18，22天重新生长速度较C组慢（T＝2，3，P＜0.05)。A组的肿瘤倍增时间较B组、C组长。病理切片显示A组肿瘤细胞坏死明显，仅有少数细胞残核，B组肿瘤细胞部分坏死，C组肿瘤细胞坏死不明显。④结论 rhEPO结合高低氧放疗对贫血荷瘤小鼠具有放射增敏的作用，值得临床推广应用。

EFFECTS OF rhEPO COMBINING CARBOGEN PLUS LOW CONCENTRATION OXYGEN ON ENHANCED RADIOSENSITIVITY FOR TUMORS IN ANEMIC MICE

Objective\ To investigate the effects of re co mbinant human erythropoietin(rhEPO) combining carbogen plus low concentration ox ygen radiotherapy (CPLCOR)on enhanced radiosensitivity for tumors in anemic mice . \ Methods\ Kun Ming mice were randomly divided into three groups: Gr oups A, B and C. The mice in Group A were pretreated with rhEPO to prevent anemi a induced by carboplatin, and then the tumors were irradiated with CPLCOR. Anemi a of the mice in Group B and Group C was induced by carboplatin. The tumors in Group B were irradiated with CPLCOR; tumors in Group C were treated with irradia tion only. The differences of effects of irradiation were investigated and biops ies were done for all experimental mice. \ Results Fourteen days after tumor implantation, the regenerati on speed of the tumors in Group A was dramatically slowed compared with that in Group B and C ( T=0-3,P <0.05). A comparison between Group B and Group C showe d that the speed of regeneration of tumors in Group B, 18 and 22 days after impl antation of the tumors, was slower than that in Group C ( T=2,3,P <0.05). The volume doubling time of tumors in group A was longer than that of the tumors in Groups B and C. The histology indicated that necrosis of tumor cells was eviden t with few residual nuclear in Group A, partial necrosis in Group B but not obvi ous in Group C. Conclusion\ The rhEPO treatment combining CPLCOR increases tumo r radiosensitivity of anemic mice and it is advocated clinically.