开心散配伍比例对慢性压力应激抑郁小鼠皮层与海马神经营养因子系统调控的影响

目的?基于慢性压力应激抑郁小鼠模型，考察不同配伍比例的开心散对皮层与海马神经营养因子调控的影响，探讨开心散治疗抑郁症的作用机制。方法?采用一系列慢性应激压力构建小鼠抑郁症模型，给予不同配伍比例的开心散，应用ELISA法分别检测小鼠大脑皮层与海马中神经生长因子NGF与脑源性神经营养因子BDNF含量，利用qPCR与Western blot技术检测皮层与海马神经营养因子受体TrkA、TrkB、TrkC的表达。结果?不同配伍比例的开心散均能提升抑郁小鼠的糖水饮用量，延长强迫游泳时间，表现出显著的抗抑郁作用，其中以D-652配伍比例效用最佳。同时提升NGF、BDNF含量与相关受体的表达。结论?开心散调控神经营养因子系统是其抗抑郁的重要作用机制。 

Research of Compatible Ratio of Kai-Xin-San on Regulation of Neurotrophic Factor System in Cortex and Hippocampus on Chronic Unpredictable Mild Stress Induced Depressive Mice

OBJECTIVE?To explore the anti-depressive effect of Kai-Xin-San (KXS) with different compatible ratio on chronic unpredictable mild stress (CUMS) treated mouse via neurotrophic factor regulation. METHODS?Depressive animal model was created by applying a series of stress procedures on mice and the depressive mice were treated with KXS with different compatible ratio. Several parameters， including sucrose consumption， forced swimming time， were applied for evaluation of the depressive effect of KXS. Afterwards， the mice were sacrificed. The cerebral cortex and hippocampus were took out for the analysis of quantity of neurotrophic factors and the expressions of neurotrophic factor receptors via ELISA， qPCR and western blot analysis. RESULTS?KXS with different compatible ratio exerted significant anti-depressive effect on CUMS treated depressive mouse model in presence of increased sucrose consumption， swimming time and open field scores. The amount of neurotrophic factors， NGF and BDNF， and the expression of the corresponding receptors， were both up-regulated after the treatment of KXS. One ratio named D-652 exerted the best effect. CONCLUSION?Neurotrophic factor regulation might be one of important active targets for anti-depression effect of KXS.