桂枝茯苓胶囊对EM模型大鼠异位内膜细胞凋亡、VEGF及血清雌孕激素的影响

目的 以自体移植法建立EM大鼠模型，观察桂枝茯苓胶囊对EM大鼠异位内膜细胞凋亡、VEGF及血清雌、孕激素含量的影响，探讨其治疗EM的效应机制。方法 采用自体移植法建立大鼠子宫内膜异位症模型，术后4周将造模成功大鼠随机分为模型组、米非司酮组、散结镇痛胶囊组、桂枝茯苓胶囊高、中、低剂量组，药物干预4周后进行大鼠异位内膜细胞凋亡TUNEL法检测，检测大鼠血清中雌、孕激素的含量，免疫组化法检测Caspase-7、VEGF、survivin表达。结果 桂枝茯苓高剂量组异位内膜细胞凋亡率显著高于模型组，桂枝茯苓高剂量组及米非司酮组异位内膜Caspase-7表达均较模型组显著升高，VEGF、survivin表达及血清雌、孕激素含量显著降低。结论 桂枝茯苓胶囊治疗EMS的疗效机制，可能与诱导异位内膜细胞凋亡、下调survivin的高表达、降低VEGF表达及调节血清中雌孕激素水平密切相关。 

Effects of Guizhi Fuling Capsule on Apoptosis of Heterotopic Endometrial Cells， VEGF and Estrogen and Progesterone in the Serum of Rat EM Model

OBJECTIVE To observe the effect of VEGF， estrogen and progesterone in serum on ectopic endometrial cell apoptosis in EMS rats and explore the mechanism of Guizhi Fuling Capsule. METHODS Establish rat endometriosis model by autologous transplantation methods， Four weeks after surgery， The rats were randomly divided into model group， mifepristone group， Sanjie analgesic capsule group， Guizhi Fuling pill high， medium and low dose group，with 15 rats per group. Four weeks after the drug intervention， the ectopic endometrial cell apoptosis was detected by TUNEL method The contents of estrogen and progesterone in serum were measured，The expression of caspase-7， VEGF and survivin was detected by immunohistochemistry. RESULTS The apoptotic rate of ectopic endometrial cells in Guizhi Fuling high dose group was significantly higher than model group，The expression of caspase-7 in ectopic endometrium of Guizhi Fuling high dose group and mifepristone group was significantly higher than model group，VEGF， survivin expression and the content of estrogen and progesterone in serum were significantly decreased. CONCLUSION The mechanism of the treatment of EMT by Guizhi Fuling Capsule， may be closely related to induced ectopic endometrial cell apoptosis and decreased endometrial VEGF expression and down-regulates the high expression of survivin and regulated levels of estrogen and progesterone in serum.