氯化铝对大鼠基底前脑内侧隔核神经元APP蛋白表达的影响

目的：研究氯化铝对大鼠基底前脑内侧隔核APP蛋白表达的影响.方法：选用雄性Wistar大鼠20只,随机分为老龄组（18个月）、老龄给药组（自18个月始给予腹腔内注射氯化铝至21个月）,21个月后进行Morris水迷宫训练及测试,观察大鼠的行为学改变.然后采用免疫组织化学SP法检测大鼠内侧隔核APP蛋白表达的变化情况.结果：老龄给药组大鼠逃避潜伏期与老龄组相比时间增加（P＜0.05）;老龄给药组内侧隔核APP蛋白阳性反应物的平均光密度值与老龄组相比显著增高（P＜0.05）.结论：老龄给药组大鼠内侧隔核APP蛋白表达明显高于老龄组,说明长期接触氯化铝可能导致基底前脑内侧隔核神经元APP蛋白表达或聚集增加.

The Effect of Aluminum Chloride on APP in Medial Septum of Aged Rat

Objective： To explore the effect of aluminum chloride on APP in medial septum of aged rats. Methods： Twenty male wister rats were randomly divided into aged group （ 18 months） and aged group with aluminum chloride （ intraperitoneal injection aluminum chloride from 18 months to 21 months）. The quantitative changes of APP in medial septum of wistar rats were studied with immunohistochemical SP method. Results： The escape latency period of aged group with aluminum chloride was increased compared with that of aged group （ P 〈 0. 05 ）. The optical density value of aged group with aluminum chloride was increased compared with that of aged group （ P 〈 0. 05 ）. Conclusion ： The level of APP expression is increased in medial septum of aged group with alumi- num chloride, so long-term exposure to aluminum chloride can result in increase for APP expression and aggregation in neurons of basal forebrain.