过量氟与大鼠骨质硬化关系的研究

目的：研究过量氟与大鼠骨质硬化发生之间的关系。方法：Wistar大鼠40只，雌雄各半，随机分为四组，其中三组分别饮用氟（F^-）浓度为25、50、100mg／L的含氟水，一组为对照组，饮用蒸馏水。3个月后采用管式炉高温燃烧水解法-氟离子选择电极法测定骨氟含量，采用氟离子选择电极法测定血清氟和尿氟含量。采用全自动生化分析仪测定血清中钙（Ca）、磷（P）及碱性磷酸酶（ALP）等指标；采用骨密度测量仪测量大鼠股骨骨密度。结果：与对照组相比，各染氟组大鼠的血清氟、骨氟、尿氟含量明显升高（P〈0．05），血清中ALP水平明显升高（P〈0．05），各染氟组大鼠骨密度显著高于对照组（P〈0．05），且骨密度和骨氟含量都密切相关（r=0．493，P〈0．01）。结论：过量氟对大鼠骨质硬化的形成发挥重要作用。

Study on Relationship between Excessive Fluoride and Osteosclerosis in Chronic Fluorosis Rats

Objective： To study the relationship between excessive fluoride and osteosclerosis in chronic fluorosis rats. Methods： Fourty Wistar rats were randomly classified into four groups. One control group was given pure water for drinking, and in other groups the fluoride was administered through drinking water with different doses of fluorin ion （25, 50, 100 mg/L F- respectively） for a period of three months. At the end of the experiment, concentrations of fluoride in serum, bone and urine was measured by fluoride ion selective electrode method, and the level of alkaline phosphatase （ALP） , calcium （Ca） , phosphorus （P） in serum were ananlysized bv using an auto analyzer. The bone mineral density were performed with dual energy X-ray absorptiometry. Results： The levels of fluoride in serum, bone, urine and ALP in serum in fluoride-treated rats were significantly higher than controls （ P 〈 0. 05 ）. The bone mineral density was increased significantly in fluoride-treated rats （ P 〈 0.05 ）. And the bone mineral density was significantly correlated with the bone fluoride level （ r = 0. 493, P 〈 0. 01 ）. Conclusion ： Excessive fluoride might play an important role in the occurrence of osteoscterosis in chronic fluorosis rats.